ALOX5 Antikörper (AA 442-590)

Produktnummer ABIN968116

Dieses Maus Monoklonal-Antikörper erkennt spezifisch ALOX5 in WB, IHC, IP und IF. Er zeigt eine Reaktivität gegenüber Human, Maus, Ratte und Huhn und wurde in 5+ Publikationen erwähnt.

Kurzübersicht für ALOX5 Antikörper (AA 442-590) (ABIN968116)

Target: ALOX5 (Arachidonate 5-Lipoxygenase (ALOX5))

Reaktivität: Human, Maus, Ratte, Huhn

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser ALOX5 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunofluorescence (IF)

Klon: 33-5

Produktdetails anti-ALOX5 Antikörper

Bindungsspezifität: AA 442-590

Kreuzreaktivität: Huhn, Ratte (Rattus), Maus

Produktmerkmale: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human 5-Lipoxygenase aa. 442-590

Isotyp: IgG1

Anwendungsinformationen

Kommentare:

Related Products: ABIN967389, ABIN968554

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store undiluted at -20°C.

Referenzen für anti-ALOX5 Antikörper (ABIN968116)

Hundley, Prasad, Beaven: "Elevated levels of cyclooxygenase-2 in antigen-stimulated mast cells is associated with minimal activation of p38 mitogen-activated protein kinase." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 167, Issue 3, pp. 1629-36, (2001) (PubMed).

Zaitsu, Hamasaki, Matsuo, Kukita, Tsuji, Miyazaki, Hayasaki, Muro, Yamamoto, Kobayashi, Ichimaru, Kohashi, Miyazaki: "New induction of leukotriene A(4) hydrolase by interleukin-4 and interleukin-13 in human polymorphonuclear leukocytes." in: Blood, Vol. 96, Issue 2, pp. 601-9, (2000) (PubMed).

Lepley, Muskardin, Fitzpatrick: "Tyrosine kinase activity modulates catalysis and translocation of cellular 5-lipoxygenase." in: The Journal of biological chemistry, Vol. 271, Issue 11, pp. 6179-84, (1996) (PubMed).

Lepley, Fitzpatrick: "5-Lipoxygenase contains a functional Src homology 3-binding motif that interacts with the Src homology 3 domain of Grb2 and cytoskeletal proteins." in: The Journal of biological chemistry, Vol. 269, Issue 39, pp. 24163-8, (1994) (PubMed).

Matsumoto, Funk, Rådmark, Höög, Jörnvall, Samuelsson: "Molecular cloning and amino acid sequence of human 5-lipoxygenase." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 85, Issue 1, pp. 26-30, (1988) (PubMed).

Details zu ALOX5

Target: ALOX5 (Arachidonate 5-Lipoxygenase (ALOX5))

Andere Bezeichnung: 5-Lipoxygenase

Hintergrund: 5-Lipoxygenase (5-LO) is the initial enzyme that converts arachidonic acid to leukotrienes, which are important inflammatory and vasoconstrictive metabolites. It is activated in response to a number of stimuli, such as differentiation and allergen challenges. The 5-LO gene, abundantly expressed in placenta, lung, and leukocytes, encodes a protein of 674 amino acids with an apparent molecular weight of 78kDa. 5-LO is a Ca2+ and ATP-dependent enzyme that translocates from the cytosol to either a nuclear or plasma membrane compartment following activation. A proline-rich domain of 5-LO (amino acids 566-577) has been identified as a binding site for the PTyr-binding protein, Grb2. This Grb2 site links tyrosine kinases with activation and redistribution of 5-LO. Furthermore, tyrosine kinase inhibitors increase the activity of 5-LO and block the enzyme's subcellular redistribution.

Molekulargewicht: 79 kDa