SNAP91 Antikörper (AA 706-896)

Produktnummer ABIN967993

Dieses Maus Monoklonal-Antikörper erkennt spezifisch SNAP91 in WB, IHC, IF und IP. Er zeigt eine Reaktivität gegenüber Human, Ratte und Maus und wurde in 5+ Publikationen erwähnt.

Kurzübersicht für SNAP91 Antikörper (AA 706-896) (ABIN967993)

Target: SNAP91 (Synaptosomal-Associated Protein 91 (SNAP91))

Reaktivität: Human, Ratte, Maus

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser SNAP91 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP)

Klon: 34-AP180

Produktdetails anti-SNAP91 Antikörper

Bindungsspezifität: AA 706-896

Kreuzreaktivität: Maus, Human

Produktmerkmale: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Rat AP180 aa. 706-896

Isotyp: IgG1

Anwendungsinformationen

Kommentare:

Related Products: ABIN968545, ABIN967389

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store undiluted at -20° C.

Referenzen für anti-SNAP91 Antikörper (ABIN967993)

Kalthoff, Groos, Kohl, Mahrhold, Ungewickell: "Clint: a novel clathrin-binding ENTH-domain protein at the Golgi." in: Molecular biology of the cell, Vol. 13, Issue 11, pp. 4060-73, (2002) (PubMed).

Drake, Traub: "Interaction of two structurally distinct sequence types with the clathrin terminal domain beta-propeller." in: The Journal of biological chemistry, Vol. 276, Issue 31, pp. 28700-9, (2001) (PubMed).

Traub, Downs, Westrich, Fremont: "Crystal structure of the alpha appendage of AP-2 reveals a recruitment platform for clathrin-coat assembly." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 96, Issue 16, pp. 8907-12, (1999) (PubMed).

Ye, Lafer: "Clathrin binding and assembly activities of expressed domains of the synapse-specific clathrin assembly protein AP-3." in: The Journal of biological chemistry, Vol. 270, Issue 18, pp. 10933-9, (1995) (PubMed).

Morris, Schröder, Plessmann, Weber, Ungewickell: "Clathrin assembly protein AP180: primary structure, domain organization and identification of a clathrin binding site." in: The EMBO journal, Vol. 12, Issue 2, pp. 667-75, (1993) (PubMed).

Details zu SNAP91

Target: SNAP91 (Synaptosomal-Associated Protein 91 (SNAP91))

Andere Bezeichnung: AP180

Hintergrund: AP180, also known as AP-3, F1-20, NP185, and pp155, is one of four assembly proteins which are involved in the organization and assembly of clathrin triskelia in clathrin-coated vesicles. Two of these proteins, AP-1 and AP-2, exist as tetramers, while AP180 and auxilin are monomeric assembly proteins. The clathrin binding potential of AP180 is defined by several regions of the molecule, including the 30 kDa N-terminal domain, a central domain, and a 58 kDa C-terminal domain. Although clathrin binding occurs throughout the molecule, only the C-terminal domain is associated with both binding of clathrin and assembly of clathrin cages. The highly acidic central domain, which contains an uncharged alanine-rich segment, is thought to impart the irregular physical properties to this protein. AP180 is the only clathrin assembly protein specific for synapses and is thought to be involved in synaptic vesicle biogenesis and recycling. Furthermore, AP180 is bound by inositol-6-phosphate which has been shown to be closely regulated in neuronal cells by external stimuli.
Synonyms: AP-3, F1-20, NP185, and pp155

Molekulargewicht: 180 kDa