BDKRB2 Antikörper (AA 350-364)

Produktnummer ABIN967978

Dieses Maus Monoklonal-Antikörper erkennt spezifisch BDKRB2 in WB, IHC, IF und IP. Er zeigt eine Reaktivität gegenüber Human und Ratte und wurde in 5+ Publikationen erwähnt.

Kurzübersicht für BDKRB2 Antikörper (AA 350-364) (ABIN967978)

Target: BDKRB2 (Bradykinin Receptor B2 (BDKRB2))

Reaktivität: Human, Ratte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser BDKRB2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP)

Klon: 20-B2 Bradykinin Receptor

Produktdetails anti-BDKRB2 Antikörper

Bindungsspezifität: AA 350-364

Kreuzreaktivität: Ratte (Rattus)

Produktmerkmale: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
5. For fluorochrome spectra and suitable instrument settings, please refer to us.

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human B2 Bradykinin Receptor aa. 350-364

Isotyp: IgG2b kappa

Anwendungsinformationen

Kommentare:

Related Products: ABIN967389

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store undiluted at -20°C.

Referenzen für anti-BDKRB2 Antikörper (ABIN967978)

Ewert, Johansson, Holm, Helander, Fandriks: "The bradykinin BK2 receptor mediates angiotensin II receptor type 2 stimulated rat duodenal mucosal alkaline secretion." in: BMC physiology, Vol. 3, pp. 1, (2003) (PubMed).

Duchene, Schanstra, Pecher, Pizard, Susini, Esteve, Bascands, Girolami: "A novel protein-protein interaction between a G protein-coupled receptor and the phosphatase SHP-2 is involved in bradykinin-induced inhibition of cell proliferation." in: The Journal of biological chemistry, Vol. 277, Issue 43, pp. 40375-83, (2002) (PubMed).

Xie, Browning, Hay, Mackman, Ye: "Activation of NF-kappa B by bradykinin through a Galpha(q)- and Gbeta gamma-dependent pathway that involves phosphoinositide 3-kinase and Akt." in: The Journal of biological chemistry, Vol. 275, Issue 32, pp. 24907-14, (2000) (PubMed).

Golser, Gorren, Leber, Andrew, Habisch, Werner, Schmidt, Venema, Mayer: "Interaction of endothelial and neuronal nitric-oxide synthases with the bradykinin B2 receptor. Binding of an inhibitory peptide to the oxygenase domain blocks uncoupled NADPH oxidation." in: The Journal of biological chemistry, Vol. 275, Issue 8, pp. 5291-6, (2000) (PubMed).

Powell, Slynn, Thomas, Hopkins, Briggs, Graham: "Human bradykinin B2 receptor: nucleotide sequence analysis and assignment to chromosome 14." in: Genomics, Vol. 15, Issue 2, pp. 435-8, (1993) (PubMed).

Details zu BDKRB2

Target: BDKRB2 (Bradykinin Receptor B2 (BDKRB2))

Andere Bezeichnung: B2 Bradykinin Receptor

Hintergrund: Bradykinin is a nine amino acid vasoactive peptide that elicits numerous physiologic responses such as vasodilation, smooth muscle spasm, and pain. Bradykinin is one of a family of such peptides called the kinins. The kinins are generated from high molecular weight precursors called kininogens from proteolysis induced by pathophysiologic conditions such as inflammation or allergy. The physiological actions of these kinins are mediated by their interaction with transmembrane receptors. There are two distinct bradykinin receptor subtypes: B1 and B2. Both are coupled to G-proteins. The B2 receptor subtype is found in healthy smooth muscle cells and neurons, whereas, the B1 receptors are only detected following tissue injury. The B2 receptor is similar in structure to other seven helix G-protein coupled receptors. Bradykinin has a relatively low affinity for B1 receptors, and interacts primarily with the B2 receptor. This interaction stimulates several second messenger systems, including inositol phospholipid hydrolysis, arachidonic acid metabolism, tyrosine phosphorylation, and membrane depolarization and hyperpolarization.

Molekulargewicht: 42 kDa

Pathways: ACE Inhibitor Pathway, Negative Regulation of intrinsic apoptotic Signaling