Kalinin B1 (AA 1009-1170) Antikörper

Produktnummer ABIN967961

Produktdetails

Target: Kalinin B1

Bindungsspezifität: AA 1009-1170

Reaktivität: Hund, Human

Wirt: Maus

Klonalität: Monoklonal

Applikation: Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)

Kreuzreaktivität: Hund

Produktmerkmale: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human Kalinin B1 aa. 1009-1170

Klon: 17-Kalinin B1

Isotyp: IgG1

Anwendungsinformationen

Kommentare:

Related Products: ABIN967389, ABIN968536

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store undiluted at -20°C.

Referenzen

Hirosaki, Tsubota, Kariya, Moriyama, Mizushima, Miyazaki: "Laminin-6 is activated by proteolytic processing and regulates cellular adhesion and migration differently from laminin-5." in: The Journal of biological chemistry, Vol. 277, Issue 51, pp. 49287-95, (2002) (PubMed).

Hirosaki, Mizushima, Tsubota, Moriyama, Miyazaki: "Structural requirement of carboxyl-terminal globular domains of laminin alpha 3 chain for promotion of rapid cell adhesion and migration by laminin-5." in: The Journal of biological chemistry, Vol. 275, Issue 29, pp. 22495-502, (2000) (PubMed).

Gerecke, Wagman, Champliaud, Burgeson: "The complete primary structure for a novel laminin chain, the laminin B1k chain." in: The Journal of biological chemistry, Vol. 269, Issue 15, pp. 11073-80, (1994) (PubMed).

Antigendetails

Target: Kalinin B1

Hintergrund: Kalinin is an epithelial cell laminin. It is a structural component of the dermal-epidermal junction of skin and basement membrane. The full molecule is composed of a 200 kDa A subunit, a 155 kDa B2 subunit, and a 140 kDa B1 subunit. Another B1k subunit with a predicted molecular weight of 126.9 kDa is encoded by a 3.9kb gene. The apparent molecular weight of Kalinin B1k is 140 kDa due to glycosylation. Its structure is divided into a long arm and a short arm domain that bind and crosslink with the other kalinin subunits.

Molekulargewicht: 140 kDa