Desmoglein (AA 705-1029) Antikörper

Produktnummer ABIN967873

Produktdetails

Target: Desmoglein

Bindungsspezifität: AA 705-1029

Reaktivität: Ratte, Blow Fly, Huhn, Hund, Human, Maus

Wirt: Maus

Klonalität: Monoklonal

Applikation: Western Blotting (WB)

Kreuzreaktivität: Maus, Ratte (Rattus), Hund, Huhn, Fruchtfliege (Drosophila melanogaster)

Produktmerkmale: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Please refer to us for technical protocols.

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human Desmoglein aa. 705-1029

Klon: 62-Desmoglein

Isotyp: IgG2b

Anwendungsinformationen

Kommentare:

Related Products: ABIN968535, ABIN967389

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store undiluted at -20°C.

Referenzen

Stewart, Barth, Nelson: "Differential regulation of endogenous cadherin expression in Madin-Darby canine kidney cells by cell-cell adhesion and activation of beta -catenin signaling." in: The Journal of biological chemistry, Vol. 275, Issue 27, pp. 20707-16, (2000) (PubMed).

Peltonen, Hentula, Hägg, Ylä-Outinen, Tuukkanen, Lakkakorpi, Rehn, Pihlajaniemi, Peltonen: "A novel component of epidermal cell-matrix and cell-cell contacts: transmembrane protein type XIII collagen." in: The Journal of investigative dermatology, Vol. 113, Issue 4, pp. 635-42, (1999) (PubMed).

Wine, Chapin: "Adhesion and signaling proteins spatiotemporally associated with spermiation in the rat." in: Journal of andrology, Vol. 20, Issue 2, pp. 198-213, (1999) (PubMed).

Mathur, Goodwin, Cowin: "Interactions of the cytoplasmic domain of the desmosomal cadherin Dsg1 with plakoglobin." in: The Journal of biological chemistry, Vol. 269, Issue 19, pp. 14075-80, (1994) (PubMed).

Wheeler, Parker, Thomas, Ataliotis, Poynter, Arnemann, Rutman, Pidsley, Watt, Rees: "Desmosomal glycoprotein DGI, a component of intercellular desmosome junctions, is related to the cadherin family of cell adhesion molecules." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 88, Issue 11, pp. 4796-800, (1991) (PubMed).

Antigendetails

Target: Desmoglein

Hintergrund: Desmoglein (Dsg1) is a transmembrane glycoprotein found in desmosome junctions. Desmosomes and adherens junctions are plasma membrane specializations involved in cell-cell adhesion. They serve as membrane anchorage domains for intermediate filaments and microfilaments, respectively. Actin microfilaments anchor at adherens junctions, which occur in many sizes, shapes, and positions. These junctions generally contain E- or N-Cadherin whose cytoplasmic region is associated with cytoplasmic proteins such as alpha- and beta- catenin, vinculin, alpha-actinin, and radixin. Intermediate filaments (IFs) are anchored at desmosomes which contain desmosome-specific cadherin family members, known as desmocollin (Dsc) and desmoglein. These proteins associate with cytoplasmic proteins such as desmoplakin and plakoglobin (gamma-Catenin). Plakoglobin is the only junctional protein found at both desmosomes and adherens junctions and, therefore, plays a crucial role in cell adhesion.
Synonyms: Dsg1

Molekulargewicht: 165 kDa