Dynamin 1 Antikörper (AA 698-851)

Produktnummer ABIN967852

Dieses Maus Monoklonal-Antikörper erkennt spezifisch Dynamin 1 in WB, IHC, IF und IP. Er zeigt eine Reaktivität gegenüber Human, Maus, Ratte, Hund und Huhn und wurde in 5+ Publikationen erwähnt.

Kurzübersicht für Dynamin 1 Antikörper (AA 698-851) (ABIN967852)

Target: Dynamin 1 (DNM1)

Reaktivität: Human, Maus, Ratte, Hund, Huhn

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Dynamin 1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP)

Klon: 41-Dynamin

Produktdetails anti-Dynamin 1 Antikörper

Bindungsspezifität: AA 698-851

Kreuzreaktivität: Human, Hund, Huhn, Maus

Produktmerkmale: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Rat Dynamin I aa. 698-851

Isotyp: IgG1

Anwendungsinformationen

Kommentare:

Related Products: ABIN968539, ABIN967389

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store undiluted at -20° C.

Referenzen für anti-Dynamin 1 Antikörper (ABIN967852)

Hayes, Chawla, Corvera: "TGF beta receptor internalization into EEA1-enriched early endosomes: role in signaling to Smad2." in: The Journal of cell biology, Vol. 158, Issue 7, pp. 1239-49, (2002) (PubMed).

Kalthoff, Groos, Kohl, Mahrhold, Ungewickell: "Clint: a novel clathrin-binding ENTH-domain protein at the Golgi." in: Molecular biology of the cell, Vol. 13, Issue 11, pp. 4060-73, (2002) (PubMed).

Schmidlin, Dery, DeFea, Slice, Patierno, Sternini, Grady, Bunnett: "Dynamin and Rab5a-dependent trafficking and signaling of the neurokinin 1 receptor." in: The Journal of biological chemistry, Vol. 276, Issue 27, pp. 25427-37, (2001) (PubMed).

Ochoa, Slepnev, Neff, Ringstad, Takei, Daniell, Kim, Cao, McNiven, Baron, De Camilli: "A functional link between dynamin and the actin cytoskeleton at podosomes." in: The Journal of cell biology, Vol. 150, Issue 2, pp. 377-89, (2000) (PubMed).

Scaife, Gout, Waterfield, Margolis: "Growth factor-induced binding of dynamin to signal transduction proteins involves sorting to distinct and separate proline-rich dynamin sequences." in: The EMBO journal, Vol. 13, Issue 11, pp. 2574-82, (1994) (PubMed).

Details zu Dynamin 1

Target: Dynamin 1 (DNM1)

Andere Bezeichnung: Dynamin

Hintergrund: Dynamin is a nerve terminal phosphoprotein with intrinsic GTPase activity which plays an important role in endocytosis. This GTPase activity is stimulated in vitro by microtubules, SH3 domain-containing proteins, phospholipids, and Protein Kinase C (PKC)-mediated phosphorylation. There are at least two distinct dynamin genes in mammals, which show 79% identity, encoding proteins Dynamin I and Dynamin II. Dynamin I is expressed almost exclusively in the central nervous system while Dynamin II expression is ubiquitous. Depolarization stimulates synaptic vesicle recycling and Dynamin I is subsequently dephosphorylated. This rapid dephosphorylation is mediated by calcineurin which acts as a switch for depolarization-initiated synaptic vesicle endocytosis. The C-terminus of dynamin contains two clusters of proline-rich SH3 domain binding proline motifs which interact with known SH3 domain proteins during tyrosine kinase receptor activation. It is reported that clone 41 detects both dynamin I and dynamin II.

Molekulargewicht: 100 kDa

Pathways: Toll-Like Receptors Cascades, CXCR4-mediated Signaling Events, Thromboxane A2 Receptor Signaling