C-Raf-1 (AA 162-378) Antikörper

Produktnummer ABIN967783

Dieses Maus Monoklonal-Antikörper erkennt spezifisch in IF, IP, IHC und WB. Er zeigt eine Reaktivität gegenüber Huhn, Hund, Human, Maus und Ratte und wurde in 5+ Publikationen erwähnt.

Kurzübersicht für C-Raf-1 (AA 162-378) Antikörper (ABIN967783)

Target: C-Raf-1

Reaktivität: Huhn, Hund, Human, Maus, Ratte

Wirt: Maus

Klonalität: Monoklonal

Applikation: Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)

Klon: 53-c-Raf

Produktdetails

Bindungsspezifität: AA 162-378

Kreuzreaktivität: Hund, Ratte (Rattus), Maus, Huhn

Produktmerkmale: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human c-Raf-1 aa. 162-378

Isotyp: IgG1

Anwendungsinformationen

Kommentare:

Related Products: ABIN968533, ABIN967389

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store undiluted at -20° C.

Referenzen

Coles, Shaw: "PAK1 primes MEK1 for phosphorylation by Raf-1 kinase during cross-cascade activation of the ERK pathway." in: Oncogene, Vol. 21, Issue 14, pp. 2236-44, (2002) (PubMed).

Rizzo, Kraft, Watkins, Levitan, Romero: "Agonist-dependent traffic of raft-associated Ras and Raf-1 is required for activation of the mitogen-activated protein kinase cascade." in: The Journal of biological chemistry, Vol. 276, Issue 37, pp. 34928-33, (2001) (PubMed).

Cheng, Wung, Chao, Wang: "Sequential activation of protein kinase C (PKC)-alpha and PKC-epsilon contributes to sustained Raf/ERK1/2 activation in endothelial cells under mechanical strain." in: The Journal of biological chemistry, Vol. 276, Issue 33, pp. 31368-75, (2001) (PubMed).

Bondzi, Grant, Krystal: "A novel assay for the measurement of Raf-1 kinase activity." in: Oncogene, Vol. 19, Issue 43, pp. 5030-3, (2000) (PubMed).

Rapp: "Role of Raf-1 serine/threonine protein kinase in growth factor signal transduction." in: Oncogene, Vol. 6, Issue 4, pp. 495-500, (1991) (PubMed).

Antigendetails

Target: C-Raf-1

Hintergrund: Raf (c-Raf-1) is a cytoplasmic serine/threonine protein kinase and is a member of a family of proteins which are highly conserved from Drosophila to mammals. Raf has a critical role in the response to many growth factors including: EGF, PDGF, insulin, IL-2, IL-3, CSF-1, and GM-CSF. Raf can directly interact with Ras-GTP and subsequently become activated. Thus Raf plays a prominent role in the Ras signaling pathway by transferring a signal from Ras which has been activated by growth factor receptor-stimulated tyrosine kinase activity. This leads to the stimulation and activation of a number of other cytoplasmic serine/threonine kinases. Raf regulates the MAP kinase pathway by phosphorylating and activating MEK, which then phosphorylates and activates MAP kinase (ERK). ERK then phosphorylates and activates Rsk. Raf activity can also be regulated independently of Ras. Cyclic AMP (cAMP) activation of protein kinase A (PKA) can inhibit growth factor stimulation of Raf.

Molekulargewicht: 74 kDa