N-Cadherin Antikörper (Extracellular Domain)

Produktnummer ABIN967678

Dieses Maus Monoklonal-Antikörper erkennt spezifisch N-Cadherin in WB, IF, FACS und BI. Er zeigt eine Reaktivität gegenüber Human und wurde in 3+ Publikationen erwähnt.

Kurzübersicht für N-Cadherin Antikörper (Extracellular Domain) (ABIN967678)

Target: N-Cadherin (CDH2) (Cadherin 2 (CDH2))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser N-Cadherin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), BioImaging (BI)

Klon: 8C11

Produktdetails anti-N-Cadherin Antikörper

Bindungsspezifität: Extracellular Domain

Marke: BD Pharmingen™

Produktmerkmale: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. An isotype control should be used at the same concentration as the antibody of interest.
3. Please refer to us for technical protocols.
4. Sodium azide is a reversible inhibitor of oxidative metabolism, therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use.
5. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human extracellular N-Cadherin domain Recombinant Protein

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: Because the extracellular domain of N-Cadherin is trypsin-sensitive, it is important to avoid using trypsin to dissociate the cells to be studied.

Kommentare:

Related Products: ABIN968535, ABIN967389

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Store undiluted at 4°C.

Referenzen für anti-N-Cadherin Antikörper (ABIN967678)

Wein, Pietsch, Saffrich, Wuchter, Walenda, Bork, Horn, Diehlmann, Eckstein, Ho, Wagner: "N-cadherin is expressed on human hematopoietic progenitor cells and mediates interaction with human mesenchymal stromal cells." in: Stem cell research, Vol. 4, Issue 2, pp. 129-39, (2010) (PubMed).

Puch, Armeanu, Kibler, Johnson, Müller, Wheelock, Klein: "N-cadherin is developmentally regulated and functionally involved in early hematopoietic cell differentiation." in: Journal of cell science, Vol. 114, Issue Pt 8, pp. 1567-77, (2001) (PubMed).

Knudsen, Soler, Johnson, Wheelock: "Interaction of alpha-actinin with the cadherin/catenin cell-cell adhesion complex via alpha-catenin." in: The Journal of cell biology, Vol. 130, Issue 1, pp. 67-77, (1995) (PubMed).

Details zu N-Cadherin

Target: N-Cadherin (CDH2) (Cadherin 2 (CDH2))

Andere Bezeichnung: CD325

Hintergrund: The 8C11 monoclonal antibody recognizes the extracellular domain of human N-Cadherin (CD325). Cadherins are a family of Ca2+ dependent intercellular adhesion molecules that play a central role in controlling morphogenetic movements during development. Their function is regulated by association with the actin cytoskeleton by a complex of cytoplasmic proteins called the catenins (alpha, beta, gamma). Members of the cadherin family include P-cadherin , E-cadherin (uvomorulin), N-cadherin (neural cadherin), R-cadherin, cadherin 5, L-CAM, and EP-cadherin. N-cadherin mRNA is found at elevated levels in brain and heart and at a much lower level in liver. Mechanisms such as mRNA expression, cytokine modulation, and protease-mediated turnover modulate N-cadherin protein levels during development. In addition, N-cadherin function is indirectly regulated by endogenous kinases and phosphatases. Tyrosine phosphorylation of beta-catenin complexed with N-cadherin results in dissociation of N-cadherin from actin. However, N-cadherin also interacts with a PTP1B-like phosphatase that dephosphorylates beta-catenin and promotes N-cadherin/actin association. Thus, N-cadherin is an integral adhesion molecule whose function is regulated by protein-protein interactions and phosphorylation/dephosphorylation events.
Synonyms: Cadherin-2, N-Cadherin

Molekulargewicht: 130 kDa

Gen-ID: 1000

Pathways: Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Synaptic Membrane