BCAR1 Antikörper (pTyr249)

Produktnummer ABIN967617

Produktdetails anti-BCAR1 Antikörper

Target: BCAR1 (Breast Cancer Anti-Estrogen Resistance 1 (BCAR1))

Bindungsspezifität: pTyr249

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser BCAR1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Marke: BD Pharmingen™

Produktmerkmale: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Phosphorylated Human p130Cas

Klon: J169-757-12-2

Isotyp: IgG2b kappa

Anwendungsinformationen

Kommentare:

Related Products: ABIN967389

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Store undiluted at 4°C.

Referenzen für anti-BCAR1 Antikörper (ABIN967617)

Goldberg, Alexander, Pellicena, Zhang, Tsuda, Miller: "Src phosphorylates Cas on tyrosine 253 to promote migration of transformed cells." in: The Journal of biological chemistry, Vol. 278, Issue 47, pp. 46533-40, (2003) (PubMed).

Hinsby, Olsen, Bennett, Mann: "Signaling initiated by overexpression of the fibroblast growth factor receptor-1 investigated by mass spectrometry." in: Molecular & cellular proteomics : MCP, Vol. 2, Issue 1, pp. 29-36, (2003) (PubMed).

Details zu BCAR1

Target: BCAR1 (Breast Cancer Anti-Estrogen Resistance 1 (BCAR1))

Andere Bezeichnung: p130 Cas (BCAR1 Produkte)

Synonyme: CAS antikoerper, CAS1 antikoerper, CASS1 antikoerper, CRKAS antikoerper, P130Cas antikoerper, AI385681 antikoerper, Cas antikoerper, Crkas antikoerper, P130CAS antikoerper, MGC68466 antikoerper, cas antikoerper, p130cas antikoerper, BCAR1 antikoerper, fc17f11 antikoerper, wu:fc17f11 antikoerper, zgc:175192 antikoerper, BCAR1, Cas family scaffolding protein antikoerper, breast cancer anti-estrogen resistance 1 antikoerper, breast cancer anti-estrogen resistance 1 L homeolog antikoerper, breast cancer anti-estrogen resistance protein 1 antikoerper, BCAR1 antikoerper, Bcar1 antikoerper, bcar1.L antikoerper, bcar1 antikoerper, LOC100435586 antikoerper

Hintergrund: P47v-crk (v-Crk) is the product of a transforming gene, v-crk, that was isolated from avian sarcoma viruses. The v-Crk protein is a fusion product of viral Gag protein and a part of cellular Crk that includes SH2 and SH3 domains. v-Crk-induced transformation increases tyrosine phosphorylation of several cellular proteins, including p130Cas (CRK-associated substrate). The p130Cas is tightly associated with v-Crk via the SH2 domain of v-Crk. Tyrosine phosphorylation of p130Cas occurs in conjunction with cellular transformation in cells that express v-Src or v-Crk. This phosphorylation leads to a change in p130Cas localization from the cytoplasm to the cell membrane and, possibly, to the nucleus. Since p130Cas also associates with v-Src, it may be a v-Src substrate. Several phosphorylation sites have been described in p130Cas upon Fibroblast Growth Factor stimulation, and phosphorylated tyrosine (Y249) might function as a binding site for the Crk-adaptor molecule.
The J169-757.12.2 monoclonal antibody recognizes the phosphorylated Y249 of human p130Cas. The orthologous phosphorylation sites in mouse and rat p130Cas are Y253 and Y347, respectively.

Molekulargewicht: 130 kDa

Pathways: EGFR Signaling Pathway, Neurotrophin Signalübertragung, CXCR4-mediated Signaling Events, Platelet-derived growth Factor Receptor Signaling