SRSF2 Antikörper

Produktnummer ABIN967516

Dieses Maus Monoklonal-Antikörper erkennt spezifisch SRSF2 in WB, IP und IF. Er zeigt eine Reaktivität gegenüber Human und wurde in 3+ Publikationen erwähnt.

Kurzübersicht für SRSF2 Antikörper (ABIN967516)

Target: SRSF2 (serine/arginine-Rich Splicing Factor 2 (SRSF2))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser SRSF2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF)

Klon: AlphaSC35

Produktdetails anti-SRSF2 Antikörper

Marke: BD Pharmingen™

Produktmerkmale: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Partially purified spliceosomes

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: Staining is nuclear, a punctate or speckled nuclear pattern may be detected. Only a subset of cells are stained, the percentage may vary between cell type and experimental conditions. MG-63 human osteosarcoma, HeLa carcinoma (ATCC CCL-2), or BRL-3A rat liver cells (ATCC CRL-1442) are suggested as positive controls.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Store undiluted at 4°C.

Referenzen für anti-SRSF2 Antikörper (ABIN967516)

Sukegawa, Blobel: "A putative mammalian RNA helicase with an arginine-serine-rich domain colocalizes with a splicing factor." in: The Journal of biological chemistry, Vol. 270, Issue 26, pp. 15702-6, (1995) (PubMed).

Fu: "Specific commitment of different pre-mRNAs to splicing by single SR proteins." in: Nature, Vol. 365, Issue 6441, pp. 82-5, (1993) (PubMed).

Fu, Maniatis: "Factor required for mammalian spliceosome assembly is localized to discrete regions in the nucleus." in: Nature, Vol. 343, Issue 6257, pp. 437-41, (1990) (PubMed).

Details zu SRSF2

Target: SRSF2 (serine/arginine-Rich Splicing Factor 2 (SRSF2))

Andere Bezeichnung: SC35

Hintergrund: SC35 belongs to a highly conserved SR splicing factor family. Members of this family have characteristic RNA-binding domains and serine/arginine-rich (SR) motifs. SR proteins are expressed in the nucleus and are essential pre-messenger RNA splicing factors. Nuclear pre-messenger RNA takes place in spliceosomes, multicomponent complexes consisting of five small nuclear ribonucleoprotein particles (U1, U2, U4, U5, U6) and other non-snRNP factors such as SC35. SC35 is necessary for an early step in spliceosome assembly and can also influence the selection of alternative splice sites. SC35 is identified as a 35 kDa protein in SDS/PAGE. Clone alphaSC35 has been shown to recognize SC35 in human, and rat cells. However, it should recognize SC35 from any species, as SC35 is conserved from Drosophila to man. Partially purified spliceosomes were used as immunogen.