CD56 Antikörper

Produktnummer ABIN967498

Produktdetails anti-CD56 Antikörper

Target: CD56 (NCAM1) (Neural Cell Adhesion Molecule 1 (NCAM1))

Reaktivität: Human, Maus, Ratte

Wirt: Ratte

Klonalität: Monoklonal

Konjugat: Dieser CD56 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro))

Marke: BD Pharmingen™

Kreuzreaktivität: Human, Ratte (Rattus)

Produktmerkmale: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Mouse membrane fractions

Klon: 12F8

Isotyp: IgM

Anwendungsinformationen

Applikationshinweise: In western blot analysis, clone 12F8 recognizes N-CAM as a broad band ranging from 90 kDa to more than 200 kDa, depending on forms present. Mouse embryonic brain is suggested as a positive control.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Store undiluted at 4°C.

Referenzen für anti-CD56 Antikörper (ABIN967498)

Hankin, Lagenaur: "Cell adhesion molecules in the early developing mouse retina: retinal neurons show preferential outgrowth in vitro on L1 but not N-CAM." in: Journal of neurobiology, Vol. 25, Issue 5, pp. 472-87, (1994) (PubMed).

Miller, Styren, Lagenaur, DeKosky: "Embryonic neural cell adhesion molecule (N-CAM) is elevated in the denervated rat dentate gyrus." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 14, Issue 7, pp. 4217-25, (1994) (PubMed).

Miller, Chung, Lagenaur, DeKosky: "Regional distribution of neural cell adhesion molecule (N-CAM) and L1 in human and rodent hippocampus." in: The Journal of comparative neurology, Vol. 327, Issue 3, pp. 341-9, (1993) (PubMed).

Walsh, Doherty: "Factors regulating the expression and function of calcium-independent cell adhesion molecules." in: Current opinion in cell biology, Vol. 5, Issue 5, pp. 791-6, (1993) (PubMed).

Chung, Lagenaur, Yan, Lund: "Developmental expression of neural cell adhesion molecules in the mouse neocortex and olfactory bulb." in: The Journal of comparative neurology, Vol. 314, Issue 2, pp. 290-305, (1992) (PubMed).

Chuang, Lagenaur: "Central nervous system antigen P84 can serve as a substrate for neurite outgrowth." in: Developmental biology, Vol. 137, Issue 2, pp. 219-32, (1990) (PubMed).

Details zu CD56

Target: CD56 (NCAM1) (Neural Cell Adhesion Molecule 1 (NCAM1))

Andere Bezeichnung: CD56 (N-CAM) (NCAM1 Produkte)

Synonyme: CD56 antikoerper, MSK39 antikoerper, NCAM antikoerper, fj56g09 antikoerper, ncam antikoerper, ncam1 antikoerper, wu:fj56g09 antikoerper, NCAM1 antikoerper, n-cam antikoerper, E-NCAM antikoerper, NCAM-1 antikoerper, Ncam antikoerper, Cd56 antikoerper, N-CAM antikoerper, N-CAM-1 antikoerper, NCAM-C antikoerper, NCAMC antikoerper, N-CAM-1-B antikoerper, NCAM-1-B antikoerper, ncam1-a antikoerper, ncam1-b antikoerper, neural cell adhesion molecule 1 antikoerper, neural cell adhesion molecule 1a antikoerper, neural cell adhesion molecule 1 S homeolog antikoerper, neural cell adhesion molecule 1 L homeolog antikoerper, NCAM1 antikoerper, ncam1a antikoerper, ncam1 antikoerper, LOC100085373 antikoerper, ncam1.S antikoerper, Ncam1 antikoerper, ncam1.L antikoerper

Hintergrund: Cell adhesion molecules are present on the surface of most cells (CAMs). Neural CAMs play a key role in the development of the embryonic nervous system. Expression of neural CAMs provides neurons with a means of attaching to and interacting with other cells and the extracellular matrix. The cell surface adhesion molecule N-CAM is important in mediating initial axon outgrowth and cell migration during development. In the mature nervous system, N-CAM is important in regulating cell interactions. Additionally, the expression of several CAMs, including N-CAM is altered following injury to the peripheral nervous system. Antibodies recognizing specific CAM forms have been instrumental in defining their roles in the nervous system. Monoclonal 12F8 specifically recognizes the polysialic acid on N-CAM. The embryonic form of N-CAM is highly polysialylated during development which is later replaced by the lesspolysialic acid (PSA)-laden mature forms of N-CAM. Clone 12F8 recognizes the polysialic acid on N-CAM.2 It recognizes all species tested, from fish through human. 12F8 reacts with a neuraminidase-sensitive epitope of N-CAM. The epitope recognized by 12F8 is more highly expressed in the embryonic than in the adult brain. As such, 12F8 is useful for distinguishing embryonic forms of N-CAM from adult forms which have decreased polysialic acid expression. In addition to brain, 12F8 reacts with some non-neuronal cells. However it does not appear to recognize mouse NK cells. Mouse membrane fractions were used as immunogen.
Synonyms: N-CAM

Molekulargewicht: 90-200 kDa