FLI1 Antikörper

Produktnummer ABIN967450

Dieses Maus Monoklonal-Antikörper erkennt spezifisch FLI1 in WB und IP. Er zeigt eine Reaktivität gegenüber Human und wurde in 2+ Publikationen erwähnt.

Kurzübersicht für FLI1 Antikörper (ABIN967450)

Target: FLI1 (Friend Leukemia Virus Integration 1 (FLI1))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser FLI1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunoprecipitation (IP)

Klon: G146-222

Produktdetails anti-FLI1 Antikörper

Marke: BD Pharmingen™

Produktmerkmale: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Fli-1 ets Domain Fusion Protein

Isotyp: IgG2b

Anwendungsinformationen

Applikationshinweise: G146-222 has been shown to recognize in vitro translated, recombinant bacterially expressed and endogenous B cell Fli-1. In gel shift assays, this antibody disrupts complexes of Fli-1 and DNA probe, however, this application is not tested.

Kommentare:

Related Products: ABIN967389

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Store undiluted at 4°C.

Referenzen für anti-FLI1 Antikörper (ABIN967450)

Bailly, Bosselut, Zucman, Cormier, Delattre, Roussel, Thomas, Ghysdael: "DNA-binding and transcriptional activation properties of the EWS-FLI-1 fusion protein resulting from the t(11;22) translocation in Ewing sarcoma." in: Molecular and cellular biology, Vol. 14, Issue 5, pp. 3230-41, (1994) (PubMed).

May, Lessnick, Braun, Klemsz, Lewis, Lunsford, Hromas, Denny: "The Ewing's sarcoma EWS/FLI-1 fusion gene encodes a more potent transcriptional activator and is a more powerful transforming gene than FLI-1." in: Molecular and cellular biology, Vol. 13, Issue 12, pp. 7393-8, (1994) (PubMed).

Details zu FLI1

Target: FLI1 (Friend Leukemia Virus Integration 1 (FLI1))

Andere Bezeichnung: Fli-1

Hintergrund: Fli-1 is a 50 kDa ets-related transcription factor. Like other ets-related proteins it binds to consensus sites in DNA through an 85 amino acid ets domain in the carboxyl region of the protein. Fli-1 is associated with Ewing sarcoma (EWS) through a t(11,22)(q24,q12) chromosomal translocation. This translocation brings the 5' region of the EWS gene into conjunction with the 3' region of the Fli-1 gene encoding the ets-DNA binding domain. Such a translocation is found in 85% of Ewing sarcomas. The resulting fusion protein has the DNA binding activity of Fli- 1 and, with the EWS domain, it is also a more potent transcriptional activator than Fli-1 itself. The strong transforming activity of the EWS-Fli-1 fusion protein may in part be due to its ability to transactivate the promoter for c-myc. G146-222 recognizes human Fli-1. The antibody was raised against a bacterially expressed Fli-1 ets domain fusion protein.

Molekulargewicht: 50 kDa