L1 Protein Antikörper

Produktnummer ABIN967423

Dieser Maus Monoklonal Antikörper detektiert spezifisch L1 Protein in IHC (f), IF und WB. Es zeigt Reaktivität gegenüber Proben von Papillomavirus. Es wurde in 4+ Publikationen zitiert.

Kurzübersicht für L1 Protein Antikörper (ABIN967423)

Target: L1 Protein

Reaktivität: Papillomavirus

Wirt: Maus

Klonalität: Monoklonal

Applikation: Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunofluorescence (IF), Western Blotting (WB)

Klon: CAMVIR

Produktdetails

Produktmerkmale: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
3. Please refer to us for technical protocols.

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Isotyp: IgG2a kappa

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Store undiluted at 4°C.

Referenzen

Doorbar, Ely, Coleman, Hibma, Davies, Crawford: "Epitope-mapped monoclonal antibodies against the HPV16E1--E4 protein." in: Virology, Vol. 187, Issue 1, pp. 353-9, (1992) (PubMed).

Zhou, Sun, Stenzel, Frazer: "Expression of vaccinia recombinant HPV 16 L1 and L2 ORF proteins in epithelial cells is sufficient for assembly of HPV virion-like particles." in: Virology, Vol. 185, Issue 1, pp. 251-7, (1991) (PubMed).

McLean, Churcher, Meinke, Smith, Higgins, Stanley, Minson: "Production and characterisation of a monoclonal antibody to human papillomavirus type 16 using recombinant vaccinia virus." in: Journal of clinical pathology, Vol. 43, Issue 6, pp. 488-92, (1990) (PubMed).

de Villiers: "Heterogeneity of the human papillomavirus group." in: Journal of virology, Vol. 63, Issue 11, pp. 4898-903, (1989) (PubMed).

Antigendetails

Target: L1 Protein

Substanzklasse: Viral Protein

Hintergrund: More than 60 different types of human papilloma viruses (HPVs) have been isolated. The human papilloma viruses encode three late proteins, which are produced only in terminally differentiating keratinocytes, two of which (the L1 and L2 proteins) are structural components of the virion. Virus-like particles can be assembled by over-expressing L1 and L2 in vitro, demonstrating that L1 and L2 are necessary and sufficient components of the HPV capsomeres. HPV-16 has been reported to migrate at a reduced molecular weight of ~57 kD by SDS-PAGE. The CAMVIR-1 antibody reacts with the L1 protein of HPV-16 and may also cross-react with other HPV types, such as HPV-33.