ITK Antikörper (AA 1-26)

Produktnummer ABIN967287

Dieses Anti-ITK-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von ITK in WB und IP. Geeignet für Human und Maus. Dieses Primary Antibody wurde in 3+ Publikationen zitiert.

Kurzübersicht für ITK Antikörper (AA 1-26) (ABIN967287)

Target: ITK (IL2-Inducible T-Cell Kinase (ITK))

Reaktivität: Human, Maus

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser ITK Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunoprecipitation (IP)

Klon: 2F12

Produktdetails anti-ITK Antikörper

Bindungsspezifität: AA 1-26

Marke: BD Pharmingen™

Produktmerkmale: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Fusion protein with aa.1-26 of human/mouse Itk

Isotyp: IgG1

Anwendungsinformationen

Kommentare:

Related Products: ABIN968537

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Store undiluted at 4°C.

Referenzen für anti-ITK Antikörper (ABIN967287)

Liu, Bunnell, Gurniak, Berg: "T cell receptor-initiated calcium release is uncoupled from capacitative calcium entry in Itk-deficient T cells." in: The Journal of experimental medicine, Vol. 187, Issue 10, pp. 1721-7, (1998) (PubMed).

Heyeck, Wilcox, Bunnell, Berg: "Lck phosphorylates the activation loop tyrosine of the Itk kinase domain and activates Itk kinase activity." in: The Journal of biological chemistry, Vol. 272, Issue 40, pp. 25401-8, (1997) (PubMed).

Siliciano, Morrow, Desiderio: "itk, a T-cell-specific tyrosine kinase gene inducible by interleukin 2." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 89, Issue 23, pp. 11194-8, (1993) (PubMed).

Details zu ITK

Target: ITK (IL2-Inducible T-Cell Kinase (ITK))

Andere Bezeichnung: ITK

Hintergrund: Itk (IL-2 inducible tyrosine kinase) is a member of the TEC family of intracellular tyrosine kinases. Members of this family are characterized by their role in hematopoietic cell signaling. Itk is expressed primarily in T cells and has been shown to play a role in T-cell antigen receptor (TCR) mediated proliferation, interleukin-2 production, and T cell differentiation. Itk, following stimulation of the TCR becomes phosphorylated on tyrosine 511 by the Src kinase Lck resulting in increased in vitro Itk kinase activity. If phenylalanine is substituted for tyrosine at position 511, the in vivo kinase activity of Itk is drastically diminished, indicating that this site plays a key role in regulating Itk function. Itk migrates at ~72 kDa on SDS/PAGE. (SWISSPROT: Q08881)

Molekulargewicht: 72 kDa

Pathways: T-Zell Rezeptor Signalweg, Fc-epsilon Rezeptor Signalübertragung