Fc gamma RII (CD32) Antikörper

Produktnummer ABIN967282

Dieses Maus Monoklonal-Antikörper erkennt spezifisch in FACS, WB, IHC (fro), BR, IHC (p) und IHC (zinc). Er zeigt eine Reaktivität gegenüber Ratte.

Kurzübersicht für Fc gamma RII (CD32) Antikörper (ABIN967282)

Target: Fc gamma RII (CD32)

Reaktivität: Ratte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Unkonjugiert

Applikation: Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Blocking Reagent (BR), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Zinc-fixed Sections) (IHC (zinc))

Klon: D34-485

Produktdetails

Marke: BD Pharmingen™

Produktmerkmale: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Sodium azide is a reversible inhibitor of oxidative metabolism, therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE™ (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use.

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Recombinant Rat CD32 Protein

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: To specifically stain cells bearing Fc gamma II receptors for flow cytometric analysis: Incubate cell suspension with this antibody (Less or equal than 1 µg/million cells) followed by an appropriate fluorochrome-conjugated second-step reagent. To reduce Fc receptor-mediated binding by antibodies of interest to FcgammaII receptor-bearing rat cells for flow cytometric analysis:
a. Preincubate cell suspension with Rat Fc Block Solution (e.g., Less or equal than 1 µg/million cells in 100 µl) at 4°C for 5 minutes.
b. Add antibody of interest directly to preincubated cells in the presence of Rat Fc Block Solution (i.e., Rat Fc Block Solution need not be washed off before staining cells)
c. If anti-Ig second-step is necessary, a reagent must be chosen which will not bind to Rat Fc Block Solution (e.g., mouse IgG1, kappa)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Store undiluted at 4° C.

Antigendetails

Target: Fc gamma RII (CD32)

Andere Bezeichnung: CD32

Hintergrund: The D34-485 antibody reacts specifically with CD32, the FcgammaII receptor. Rat CD32 is expressed on B lymphocytes, myeloid cells, and some lymphocytes in the thymic medulla. D34-485 mAb blocks binding of aggregated immunoglobulins to the FcgammaIi receptors in vitro. This antibody is routinely tested by flow cytometric analysis.
Synonyms: FcgammaII Receptor