SCPA (C-Term) Antikörper

Produktnummer ABIN967004

Es sind 5+ Publikationen für dieses Produkt verfügbar. Der Kaninchen Polyklonal anti-SCPA Antikörper wird verwendet zum Nachweis von SCPA in Proben von Staphylococcus aureus. Er wurde validiert für IHC.

Kurzübersicht für SCPA (C-Term) Antikörper (ABIN967004)

Target: SCPA

Reaktivität: Staphylococcus aureus

Wirt: Kaninchen

Klonalität: Polyklonal

Applikation: Immunohistochemistry (IHC)

Produktdetails

Bindungsspezifität: C-Term

Immunogen: Polyclonal antibody produced in rabbits immunizing with a synthetic peptide corresponding to C-terminal residues of Bacteria: Staphylococcus aureus scpA (Staphylococcal cysteine proteinaseA)

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Referenzen

Rodrigues Lopes, Alcantara, Silva, Josse, Vega, Cabrerizo, Bonhomme, Lopez, Laurent, Vandenesch, Mano, Eulalio: "Microscopy-based phenotypic profiling of infection by Staphylococcus aureus clinical isolates reveals intracellular lifestyle as a prevalent feature." in: Nature communications, Vol. 13, Issue 1, pp. 7174, (2022) (PubMed).

Stelzner, Boyny, Hertlein, Sroka, Moldovan, Paprotka, Kessie, Mehling, Potempa, Ohlsen, Fraunholz, Rudel: "Intracellular Staphylococcus aureus employs the cysteine protease staphopain A to induce host cell death in epithelial cells." in: PLoS pathogens, Vol. 17, Issue 9, pp. e1009874, (2021) (PubMed).

Golonka, Filipek, Sabat, Sinczak, Potempa: "Genetic characterization of staphopain genes in Staphylococcus aureus." in: Biological chemistry, Vol. 385, Issue 11, pp. 1059-67, (2004) (PubMed).

Dubin, Krajewski, Popowicz, Stec-Niemczyk, Bochtler, Potempa, Dubin, Holak: "A novel class of cysteine protease inhibitors: solution structure of staphostatin A from Staphylococcus aureus." in: Biochemistry, Vol. 42, Issue 46, pp. 13449-56, (2003) (PubMed).

Potempa, Dubin, Korzus, Travis: "Degradation of elastin by a cysteine proteinase from Staphylococcus aureus." in: The Journal of biological chemistry, Vol. 263, Issue 6, pp. 2664-7, (1988) (PubMed).

Antigendetails

Target: SCPA

Hintergrund: The scpA (Staphylococcal cysteine proteinaseA) is able to degrade elastin. scpA (Staphylococcal cysteine proteinaseA) has a broad endopeptidase action on proteins including elastin, but rather limited hydrolysis of small-molecule substrates. Assays are conveniently made with hemoglobin, casein or Z-Phe-Arg-NHMec as substrate. Prematurely activated/folded staphopain A is inhibited by staphostatin A (scpB), which is probably required to protect staphylococcal cytoplasmic proteins from degradation by scpA. In the cytoplasm, prematurely activated/folded scpA forms a stable non-covalent complex with scpB. Cleavage leads to the activation of scpA probably by an auto-catalytic manner. The catalytic maturation of scpA appears to reside outside the cascade of activation started by the metalloprotease aureolysin (aur) scpA (Staphylococcal cysteine proteinaseA) belongs to the peptidase C47 family.
Synonyms: sspP, sspB2, Staphopain A