MUC5AC Antikörper

Produktnummer ABIN966608

Der Maus Monoklonal Anti-MUC5AC-Antikörper wurde für ELISA, IHC (p) und IHC (fro) validiert. Er ist geeignet, MUC5AC in Proben von Human, Maus, Ratte, Affe, Kaninchen, Huhn, Schwein und Hedgehog zu detektieren. Es sind 3+ Publikationen verfügbar.

Kurzübersicht für MUC5AC Antikörper (ABIN966608)

Target: MUC5AC (Mucin 5AC, Oligomeric Mucus/gel-Forming (MUC5AC))

Reaktivität: Human, Maus, Ratte, Affe, Kaninchen, Huhn, Schwein, Hedgehog

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser MUC5AC Antikörper ist unkonjugiert

Applikation: ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Klon: B466(45M1)

Produktdetails anti-MUC5AC Antikörper

Spezifität: This antibody reacts with the peptide core of gastric mucin (MUC5AC).

Aufreinigung: Concentrated.

Immunogen: Ovarian cyst mucin.

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Immunohistochemistry: 1:50-1:100
ELISA 1:500-1:2000 (not purified can not be used to coat ELISA plates).
Staining Procedure: This antibody can be used on frozen and formalin-fixed, paraffin-embedded tissue sections. For consistant and better results, use protease treatment with formalin fixed tissues. Prolonged-fixation in buffered formalin can destroy the epitope. The antibody may be used at a dilution of 1:50-1:100 in IHC. The optimal conditions should be determined by the individual laboratory.

Kommentare:

Cellular Localization: cytoplasmic and cell surface.
Recommended Positive Control: Colon or Stomach Tissue

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.3 mg/mL

Buffer: 20 mM tris-borate, 150 mM Sodium Chloride, dialyzed media RPMI 1640/D-MEM containing fetal bovine serum, BMC-6 carrier polysaccharides, carrier protein, pH 7.5

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Referenzen für anti-MUC5AC Antikörper (ABIN966608)

Rickert, Piller, Henkel, Fromme, Lieleg: "Multifunctional glycoprotein coatings improve the surface properties of highly oxygen permeable contact lenses." in: Biomaterials advances, Vol. 145, pp. 213233, (2022) (PubMed).

Rickert, Lutz, Marczynski, Lieleg: "Several Sterilization Strategies Maintain the Functionality of Mucin Glycoproteins." in: Macromolecular bioscience, pp. e2000090, (2020) (PubMed).

Lutz, Marczynski, Grill, Wall, Lieleg: "Repulsive Backbone-Backbone Interactions Modulate Access to Specific and Unspecific Binding Sites on Surface-Bound Mucins." in: Langmuir : the ACS journal of surfaces and colloids, (2020) (PubMed).

Details zu MUC5AC

Target: MUC5AC (Mucin 5AC, Oligomeric Mucus/gel-Forming (MUC5AC))

Andere Bezeichnung: MUC5AC

Hintergrund: Peptide core of gastric mucin M1, Epitope destroyed by beta-mercaptoethanol and proteases but not by periodate (3).

Molekulargewicht: >1000kDa

Gen-ID: 4586