MUC5AC Antikörper

Produktnummer ABIN966608

Der Maus Monoklonal Anti-MUC5AC Antikörper wurde für ELISA, IHC (p) und IHC (fro) validiert. Er ist geeignet, MUC5AC in Proben von Human, Maus, Ratte, Affe, Kaninchen, Huhn, Schwein und Hedgehog zu detektieren. Es sind 3+ Publikationen verfügbar.

Kurzübersicht für MUC5AC Antikörper (ABIN966608)

Target: MUC5AC (Mucin 5AC, Oligomeric Mucus/gel-Forming (MUC5AC))

Reaktivität: Human, Maus, Ratte, Affe, Kaninchen, Huhn, Schwein, Hedgehog

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser MUC5AC Antikörper ist unkonjugiert

Applikation: ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Klon: B466(45M1)

Produktdetails anti-MUC5AC Antikörper

Spezifität: This antibody reacts with the peptide core of gastric mucin (MUC5AC).

Aufreinigung: Concentrated.

Immunogen: Ovarian cyst mucin.

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Immunohistochemistry: 1:50-1:100
ELISA 1:500-1:2000 (not purified can not be used to coat ELISA plates).
Staining Procedure: This antibody can be used on frozen and formalin-fixed, paraffin-embedded tissue sections. For consistant and better results, use protease treatment with formalin fixed tissues. Prolonged-fixation in buffered formalin can destroy the epitope. The antibody may be used at a dilution of 1:50-1:100 in IHC. The optimal conditions should be determined by the individual laboratory.

Kommentare:

Cellular Localization: cytoplasmic and cell surface.
Recommended Positive Control: Colon or Stomach Tissue

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.3 mg/mL

Buffer: 20 mM tris-borate, 150 mM Sodium Chloride, dialyzed media RPMI 1640/D-MEM containing fetal bovine serum, BMC-6 carrier polysaccharides, carrier protein, pH 7.5

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Referenzen für anti-MUC5AC Antikörper (ABIN966608)

Rickert, Piller, Henkel, Fromme, Lieleg: "Multifunctional glycoprotein coatings improve the surface properties of highly oxygen permeable contact lenses." in: Biomaterials advances, Vol. 145, pp. 213233, (2022) (PubMed).

Rickert, Lutz, Marczynski, Lieleg: "Several Sterilization Strategies Maintain the Functionality of Mucin Glycoproteins." in: Macromolecular bioscience, pp. e2000090, (2020) (PubMed).

Lutz, Marczynski, Grill, Wall, Lieleg: "Repulsive Backbone-Backbone Interactions Modulate Access to Specific and Unspecific Binding Sites on Surface-Bound Mucins." in: Langmuir : the ACS journal of surfaces and colloids, (2020) (PubMed).

Details zu MUC5AC

Target: MUC5AC (Mucin 5AC, Oligomeric Mucus/gel-Forming (MUC5AC))

Andere Bezeichnung: MUC5AC

Hintergrund: Peptide core of gastric mucin M1, Epitope destroyed by beta-mercaptoethanol and proteases but not by periodate (3).

Molekulargewicht: >1000kDa

Gen-ID: 4586