MTDH Antikörper

Produktnummer ABIN966573

Dieses Anti-MTDH-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von MTDH in WB, IHC, ELISA und FACS. Geeignet für Human. Dieses Primary Antibody wurde in 4+ Publikationen zitiert.

Kurzübersicht für MTDH Antikörper (ABIN966573)

Target: MTDH (Metadherin (MTDH))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser MTDH Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Flow Cytometry (FACS)

Klon: 2F11C3

Produktdetails anti-MTDH Antikörper

Aufreinigung: Antibodies are purified by protein G affinity chromatography.

Immunogen: Purified recombinant fragment of human Metadherin expressed in E. Coli.

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Western Bloting: 1/500 - 1/2000.
Immunohistochemistry: 1/200 - 1/1000.
Flow cytometry: 1/200 - 1/400.
ELISA: Propose dilution 1/10000.
Not yet tested in other applications.
Determining optimal working dilutions by titration test.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: PBS containing 50% glycerol

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Referenzen für anti-MTDH Antikörper (ABIN966573)

Hu, Chong, Yang, Wei, Blanco, Li, Reiss, Au, Haffty, Kang: "MTDH activation by 8q22 genomic gain promotes chemoresistance and metastasis of poor-prognosis breast cancer." in: Cancer cell, Vol. 15, Issue 1, pp. 9-20, (2008) (PubMed).

Méndez, Audicana, Cancer, Isern, Llaneza, Moreno, Navarro, Tarancón, Valero, Ribas, Jofre, Lucena: "Assessment of drinking water quality using indicator bacteria and bacteriophages." in: Journal of water and health, Vol. 2, Issue 3, pp. 201-14, (2004) (PubMed).

Britt, Yang, Yang, Flanagan, Callanan, Lim, Lin, Hixson: "Identification of a novel protein, LYRIC, localized to tight junctions of polarized epithelial cells." in: Experimental cell research, Vol. 300, Issue 1, pp. 134-48, (2004) (PubMed).

Brown, Ruoslahti: "Metadherin, a cell surface protein in breast tumors that mediates lung metastasis." in: Cancer cell, Vol. 5, Issue 4, pp. 365-74, (2004) (PubMed).

Details zu MTDH

Target: MTDH (Metadherin (MTDH))

Andere Bezeichnung: Metadherin

Hintergrund: Metadherin (Metastasis adhesion protein), also known as MTDH, LYsine-RIch CEACAM1 co-isolated (LYRIC), is a novel protein that localizes with the tight junction proteins ZO-1 and occludin in polarized epithelial cells. At the tight junction, it acts not as a structural component, but is rather recruited during the maturation of the tight junction complex. Metadherin is overexpressed in breast cancer tissue and breast tumor xenografts, while much lower levels are expressed in normal breast tissue. Metadherin binds to lung vasculature, one of the four common sites of breast cancer metastasis, through a C-terminal segment in the extracellular domain, blocking this lung-homing domain with antibodies or inhibiting metadherin with siRNA has been reported to inhibit breast cancer metastasis.
Synonyms: 3D3, AEG1, LYRIC, MTDH

Molekulargewicht: 64kDa

Gen-ID: 92140

Pathways: Cellular Response to Molecule of Bacterial Origin, Cell-Cell Junction Organization