Western Blot: 1: 500- 1: 2,000 ELISA: Propose dilution 1: 10,000. Determining optimal working dilutions by titration test.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Liquid
Lagerung
-20 °C
Mingjian, Wheeler, Cheng, Yonghai, Chen, Fitzwarryne, Wang: "HIV/AIDS prevalence and behaviour in drug users and pregnant women in Kashgar Prefecture: case report." in: Harm reduction journal, Vol. 3, pp. 7, (2006) (PubMed).
Stachlewitz, Hart, Bettencourt, Kebede, Schwartz, Ratnofsky, Calderwood, Waegell, Hirst: "A-770041, a novel and selective small-molecule inhibitor of Lck, prevents heart allograft rejection." in: The Journal of pharmacology and experimental therapeutics, Vol. 315, Issue 1, pp. 36-41, (2005) (PubMed).
Hawash, Kesavan, Magee, Geahlen, Harrison: "The Lck SH3 domain negatively regulates localization to lipid rafts through an interaction with c-Cbl." in: The Journal of biological chemistry, Vol. 277, Issue 7, pp. 5683-91, (2002) (PubMed).
Target
LCK
(Lymphocyte-Specific Protein tyrosine Kinase (LCK))
Lck(lymphocyte-specific protein tyrosine kinase), with 509-amino acid protein (about 56kDa), belongs to the Src non-receptor tyrosine kinases family. By virtue of common structural motifs, the Src family is composed of nine members in vertebrates, including Src, Yes, Fgr, Frk, Fyn, Lyn, Hck, Lck and Blk. Lck is expressed predominantly in T cells and is localized to the inner surface of the plasma membrane. Lck is activated after T cell stimulation and is required for T-cell proliferation and IL-2 production. Aberrant expression or activation of Lck kinase has been reported in both lymphoid and nonlymphoid malignancies. In addition, inhibition of Lck has been a target to prevent lymphocyte activation and acute rejection.