GNRHR Antikörper (Extracellular Domain)

Produktnummer ABIN966217

Dieses Maus Monoklonal-Antikörper erkennt spezifisch GNRHR in WB, ELISA, FACS und IHC (fro). Er zeigt eine Reaktivität gegenüber Human und wurde in 4+ Publikationen erwähnt.

Kurzübersicht für GNRHR Antikörper (Extracellular Domain) (ABIN966217)

Target: GNRHR (Gonadotropin-Releasing Hormone Receptor (GNRHR))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser GNRHR Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))

Klon: B470

Produktdetails anti-GNRHR Antikörper

Bindungsspezifität: AA 1-29, Extracellular Domain

Spezifität: This antibody reacts with GnrH receptors found in the anterior pituitary. GnrH functions as a stimulator of gonadotrophs to release luteinizing hormone (LH) and follicle stimulating hormone (FSH).

Aufreinigung: Concentrated.

Immunogen: BSA-conjugated peptide (amino acids1-29) of human GnrH receptor extracellular domain.

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Immunohistochemistry: 1:50-1:100.
ELISA: 1:1000-1:3000.
Flow cytometry: 1:150-1:300.
Western Blot 1:200-1:500.
Staining Procedure: This antibody can be used on frozen tissue sections. The antibody may be used at a dilution of 1:50-1:100 in IHC. The optimal conditions should be determined by the individual laboratory.

Kommentare:

Recommended Positive Control: Pituitary Tissue.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.3 mg/ml.

Buffer: 20 mM tris-borate, 150 mM Sodium Chloride, dialyzed media RPMI 1640/D-MEM containing fetal bovine serum, BMC-6 carrier polysaccharides, carrier protein, pH 7.5

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Referenzen für anti-GNRHR Antikörper (ABIN966217)

Grosse, Schöneberg, Schultz, Gudermann: "Inhibition of gonadotropin-releasing hormone receptor signaling by expression of a splice variant of the human receptor." in: Molecular endocrinology (Baltimore, Md.), Vol. 11, Issue 9, pp. 1305-18, (1997) (PubMed).

Jennes, Eyigor, Janovick, Conn: "Brain gonadotropin releasing hormone receptors: localization and regulation." in: Recent progress in hormone research, Vol. 52, pp. 475-90; discussion 490-1, (1997) (PubMed).

Fraser, Sellar, Illingworth, Eidne: "GnRH receptor mRNA expression by in-situ hybridization in the primate pituitary and ovary." in: Molecular human reproduction, Vol. 2, Issue 2, pp. 117-21, (1997) (PubMed).

Arora, Cheng, Catt: "Mutations of the conserved DRS motif in the second intracellular loop of the gonadotropin-releasing hormone receptor affect expression, activation, and internalization." in: Molecular endocrinology (Baltimore, Md.), Vol. 11, Issue 9, pp. 1203-12, (1997) (PubMed).

Details zu GNRHR

Target: GNRHR (Gonadotropin-Releasing Hormone Receptor (GNRHR))

Andere Bezeichnung: GnRH Receptor

Gen-ID: 2798