GAPDH Antikörper (N-Term)

Produktnummer ABIN966181

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch GAPDH in WB, ICC, IHC (p) und IHC (f). Es zeigt Reaktivität gegenüber Proben von Human. Es wurde in 2+ Publikationen zitiert.

Kurzübersicht für GAPDH Antikörper (N-Term) (ABIN966181)

Target: GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser GAPDH Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Produktdetails anti-GAPDH Antikörper

Bindungsspezifität: N-Term

Kreuzreaktivität: Maus, Ratte (Rattus)

Kreuzreaktivität (Details): No cross reactivity with other proteins.

Produktmerkmale: Rabbit IgG polyclonal antibody for Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) detection. Tested with WB, IHC-P, IHC-F, ICC in Human, Mouse, Rat.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminal of human GAPDH , identical to the related mouse and rat sequences.< br/>Immunogen was affinity purified.

Isotyp: IgG

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 µg/mL.

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Konservierungsmittel: Sodium azide, Thimerosal (Merthiolate)

Handhabung: Avoid repeated freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: At -20 °C for one year. After reconstitution, at 4 °C for one month. It can also be aliquotted and stored frozen at -20 °C for a longer time.

Referenzen für anti-GAPDH Antikörper (ABIN966181)

Colell, Ricci, Tait, Milasta, Maurer, Bouchier-Hayes, Fitzgerald, Guio-Carrion, Waterhouse, Li, Mari, Barbry, Newmeyer, Beere, Green: "GAPDH and autophagy preserve survival after apoptotic cytochrome c release in the absence of caspase activation." in: Cell, Vol. 129, Issue 5, pp. 983-97, (2007) (PubMed).

Benham, Povey: "Members of the human glyceraldehyde-3-phosphate dehydrogenase-related gene family map to dispersed chromosomal locations." in: Genomics, Vol. 5, Issue 2, pp. 209-14, (1989) (PubMed).

Details zu GAPDH

Target: GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))

Andere Bezeichnung: Glyceraldehyde-3-phosphate dehydrogenase(GAPDH)

Hintergrund: Glyceraldehyde-3-phosphate dehydrogenase catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The enzyme is thought to be a tetramer of identical chains. Several highly homologous glyceraldehyde-3-phosphate dehydrogenase (GAPD)-related sequences have been identified previously in human DNA by Southern blot analysis. Protein studies have identified only a single expressed locus for this major glycolytic enzyme, and this maps to chromosome 12p13. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a critical regulator of CICD, it mediates an elevation in glycolysis and enhanced autophagy that cooperate to protect cells from CICD.