Estrogen Receptor alpha Antikörper (AA 410-592)

Produktnummer ABIN966081

Produktdetails anti-Estrogen Receptor alpha Antikörper

Target: Estrogen Receptor alpha (ESR1) (Estrogen Receptor 1 (ESR1))

Bindungsspezifität: AA 410-592

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Estrogen Receptor alpha Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Aufreinigung: Purified

Immunogen: Purified recombinant fragment of human ER-alpha (410-592aa) expressed in E. Coli.

Klon: 2B7

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Western Bloting: 1/500 - 1/2000.
Immunohistochemistry: 1/200 - 1/1000.
ELISA: Propose dilution 1/10000.
Not yet tested in other applications.
Determining optimal working dilutions by titration test.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: PBS

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Referenzen für anti-Estrogen Receptor alpha Antikörper (ABIN966081)

Pertschuk, Feldman, Kim, Braithwaite, Schneider, Braverman, Axiotis: "Estrogen receptor immunocytochemistry in paraffin embedded tissues with ER1D5 predicts breast cancer endocrine response more accurately than H222Sp gamma in frozen sections or cytosol-based ligand-binding assays." in: Cancer, Vol. 77, Issue 12, pp. 2514-9, (1996) (PubMed).

Pertschuk, Masood, Simone, Feldman, Fruchter, Axiotis, Greene: "Estrogen receptor immunocytochemistry in endometrial carcinoma: a prognostic marker for survival." in: Gynecologic oncology, Vol. 63, Issue 1, pp. 28-33, (1996) (PubMed).

Details zu Estrogen Receptor alpha

Target: Estrogen Receptor alpha (ESR1) (Estrogen Receptor 1 (ESR1))

Andere Bezeichnung: ER-alpha (ESR1 Produkte)

Hintergrund: The estrogen receptor (ER) is a ligand-activated transcription factor composed of several domains important for hormone binding, DNA binding, and activation of transcription. Alternative splicing results in several ER mRNA transcripts, which differ primarily in their 5-prime untranslated regions. Two isoforms of the human ER, ERA and ER-beta, occur, each with distinct tissue and cell patterns of expression. Pelletier and El-Alfy (2000) studied the immunocytochemical localization of ESRA and ESRB in human reproductive tissues. In the ovary, ERB immunoreactivity was found in nuclei of granulosa cells of growing follicles at all stages from primary to mature follicles, interstitial gland, and germinal epithelium cells. Nuclear staining for ERA occurred in thecal, interstitial gland, and germinal epithelium cells. In the uterus, strong ERA immunoreactivity was detected in nuclei of epithelial, stromal, and muscle cells.
Synonyms: ER, ESR1, Era, ESRA

Gen-ID: 2099

Pathways: Nuclear Receptor Transcription Pathway, EGFR Signaling Pathway, Retinoic Acid Receptor Signaling Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway, Ribonucleoprotein Complex Subunit Organization, Ribosome Assembly