CD74, MHC CLASS11 INVARIANT CHAIN Antikörper

Produktnummer ABIN965816

Der Maus Monoklonal Anti--Antikörper wurde für IHC (fro), FACS, IF und WB validiert. Er ist geeignet, in Proben von Human zu detektieren.

Kurzübersicht für CD74, MHC CLASS11 INVARIANT CHAIN Antikörper (ABIN965816)

Target: CD74, MHC Class 11 invariant Chain

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Applikation: Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS), Immunofluorescence (IF), Western Blotting (WB)

Klon: B318 (LN-2)

Produktdetails

Spezifität: This antibody reacts with several isoforms of the CD74 antigen, the invariant chain of HLA-DR.

Aufreinigung: Purified.

Immunogen: Cell extracts from diffuse histolytic lymphoma SU-DHL-4 cells.

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Immunofluorescence: 10-20 ug/mL.
Flow cytometry: 1-5 ug/10^6 cells.
Immunoblotting: 1-5 ug/mL.
Immunohistochemistry: 10-20 ug/mL on frozen or formalin fixed paraffin embedded tissue sections using avidin-biotin system.
This product was not quality controlled in flow cytometry.
Staining Procedure: This antibody can be used on frozen cryostat sections as well as formalin-fixed, paraffin-embedded tissue sections. Prolonged fixation is buffered formalin can destroy the epitope. The antibody may be used at a dilution of 10-20 ug/mL in IHC. The optimal conditions should be determined by the individual laboratory. When using paraffin-embedded and formalin-fixed tissues, high temperature antigenic unmasking technique (see instructions below) is strongly recommended for consistent and reproducible results This antibody can be used on frozen cryostat sections as well as formalin-fixed, paraffin-embedded tissue sections. Prolonged fixation is buffered formalin can destroy the epitope. The antibody may be used at a dilution of 10-20 ug/mL in IHC. The optimal conditions should be determined by the individual laboratory. When using paraffin-embedded and formalin-fixed tissues, high temperature antigenic unmasking technique is strongly recommended for consistent and reproducible results

Kommentare:

Recommended Positive Control: Tonsil

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.4 mg/ml.

Buffer: 50 mM Sodium Borate, 150 mM Sodium Chloride, 20% Glycerol, pH 8.0

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Antigendetails

Target: CD74, MHC Class 11 invariant Chain

Andere Bezeichnung: CD74,MHC Class11 Invariant Chain

Gen-ID: 972