TNNC1 Antikörper

Produktnummer ABIN965749

Produktdetails anti-TNNC1 Antikörper

Target: TNNC1 (Cardiac Troponin C (TNNC1))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser TNNC1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)

Spezifität: This antibody is specific for human cardiac troponin subunit I, a 21 kD protein responsible for inhibiting actomyosin Mg2+-ATPase activity. This antibody has less than 0.1% reactivity with human skeletal TNI as measured by ELISA.

Produktmerkmale: High temp. antigen demasking for FFPE tissue sections. Deparaffinize and rehydrate tissue sections.
10mM citrate buffer: pH 6.0. Boil 10-20 mins, followed by cooling 10-20 mins
OR Process tissue sections in Pressure cooker for 5 mins in citrate buffer followed by cooling 10-20 mins to RT. Wash tissue sections with buffer (PBS or Automation buffer,)

Aufreinigung: Concentrated.

Immunogen: Purified human cardiac troponin I.

Klon: 2D5

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: Immunohistochemistry:1:25. For formalin-fixed paraffin-embedded (FFPE) tissue sections antigen demasker (10 mM citrate buffer pH 6.0 ) is required. Boil the tissue 10-20 mins in this buffer, followed by cooling to RT.
Western blotting: 1:50-1:100.
ELISA: 1:500-1:1000
Immunoprecipitation.
Staining Procedure: This antibody can be used on formalin-fixed, paraffin-embedded tissue sections. Prolonged fixation in buffered formalin can destroy the epitope. The antibody may be used at a dilution of 1:25-1:50 in IHC. When using paraffin-embedded and formalin-fixed tissues, high temperature antigenic unmasking technique is strongly recommended for consistent and reproducible results. This product can also be used on frozen tissue sections. The optimal conditions should be determined by the individual laboratory.

Kommentare:

Cellular Localization: cytoplasmic staining of cardiac muscle cells.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.4 mg/ml.

Buffer: 20 mM tris-borate, 150 mM Sodium Chloride, BMC-6 carrier polysaccharides, pH 7.5

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Details zu TNNC1

Target: TNNC1 (Cardiac Troponin C (TNNC1))

Andere Bezeichnung: Cardiac Troponin-1 (TNNC1 Produkte)

Synonyme: CMD1Z antikoerper, CMH13 antikoerper, TN-C antikoerper, TNC antikoerper, TNNC antikoerper, cTnC antikoerper, tnnc1 antikoerper, zgc:103465 antikoerper, PCTNC1 antikoerper, cmd1z antikoerper, cmh13 antikoerper, tnc antikoerper, tnnc antikoerper, MGC80066 antikoerper, TNNC1 antikoerper, AI874626 antikoerper, TnC antikoerper, cTnI antikoerper, tncc antikoerper, Tncc antikoerper, stnnc antikoerper, zgc:86932 antikoerper, troponin C1, slow skeletal and cardiac type antikoerper, troponin C type 1a (slow) antikoerper, troponin C type 1 (slow) L homeolog antikoerper, cardiac troponin C antikoerper, troponin C, cardiac/slow skeletal antikoerper, troponin C type 1b (slow) antikoerper, TNNC1 antikoerper, tnnc1a antikoerper, tnnc1.L antikoerper, tnnc1 antikoerper, Tnnc1 antikoerper, tnnc1b antikoerper

Molekulargewicht: 21 kDa

Gen-ID: 7134