BCL Antikörper

Produktnummer ABIN965648

Der Kaninchen Polyklonal Anti--Antikörper wurde für IHC validiert. Er ist geeignet, in Proben von Human zu detektieren. Es sind 5+ Publikationen verfügbar.

Kurzübersicht für BCL Antikörper (ABIN965648)

Target: BCL

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Applikation: Immunohistochemistry (IHC)

Produktdetails

Spezifität: BCL-2 (Ab-56) antibody detects endogenous levels of total BCL-2 protein.

Aufreinigung: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: This antiserum was produced against synthesized non-phosphopeptide derived from human BCL-2 around the phosphorylation site of threonine 56 (G-H-TP-P-H).

Anwendungsinformationen

Applikationshinweise: WB: 1:500~1:1000
IHC: 1:50~1:100.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 100ug/100ul.

Buffer: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 50% glycerol

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Referenzen

Huang, Cidlowski: "Phosphorylation status modulates Bcl-2 function during glucocorticoid-induced apoptosis in T lymphocytes." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 16, Issue 8, pp. 825-32, (2002) (PubMed).

Deng, Xiao, Lang, Gao, Ruvolo, May: "Novel role for JNK as a stress-activated Bcl2 kinase." in: The Journal of biological chemistry, Vol. 276, Issue 26, pp. 23681-8, (2001) (PubMed).

Yamamoto, Ichijo, Korsmeyer: "BCL-2 is phosphorylated and inactivated by an ASK1/Jun N-terminal protein kinase pathway normally activated at G(2)/M." in: Molecular and cellular biology, Vol. 19, Issue 12, pp. 8469-78, (2000) (PubMed).

Siegert, Robbins: "Rb inhibits the intrinsic kinase activity of TATA-binding protein-associated factor TAFII250." in: Molecular and cellular biology, Vol. 19, Issue 1, pp. 846-54, (1999) (PubMed).

Ling, Tornos, Perez-Soler: "Phosphorylation of Bcl-2 is a marker of M phase events and not a determinant of apoptosis." in: The Journal of biological chemistry, Vol. 273, Issue 30, pp. 18984-91, (1998) (PubMed).

Antigendetails

Target: BCL