Surface Lipoprotein P27 (BB_A60) Antikörper

Produktnummer ABIN964724

Hersteller Produkt- Nr.: 200-401-c30

Produktdetails

Target: Surface Lipoprotein P27 (BB_A60)

Reaktivität: Borrelia burgdorferi

Wirt: Kaninchen

Klonalität: Polyklonal

Applikation: ELISA, Western Blotting (WB)

Hersteller Produkt- Nr.: 200-401-c30

Hersteller: Rockland

Verwendungszweck: Surface Lipoprotein p27 Antibody

Kreuzreaktivität (Details): It is directed against, and shows specific reactivity for, Borrelia burgdorferi p27 protein.

Produktmerkmale: Synonyms: rabbit anti-Surface Lipoprotein p27 Antibody, BBA060 protein, Borrelia burgdorferi p27

Aufreinigung: This antibody was purified from monospecific antiserum by protein-A purified immunoaffinity chromatography, and cross-adsorbed against MBP.

Immunogen:

Immunogen: MBP-fusion protein corresponding to Borrelia burgdorferi Surface Lipoprotein p27 protein.

Immunogen Type: Recombinant Protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Application Note: Anti-Surface Lipoprotein p27 antibody has been tested in ELISA and Western Blot. Specific conditions for reactivity should be optimized by the end user. Expect a band at ~30.9 kDa in size corresponding to p27 by Western blotting in the appropriate cell lysate or extract.

Western Blot Dilution: 1:1,000

ELISA Dilution: 1:1,000

Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Konzentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Antigendetails

Target: Surface Lipoprotein P27 (BB_A60)

Andere Bezeichnung: BB_A60

Hintergrund: Background: Surface Lipoprotein p27 of Borrelia burgdorferi is a surface-exposed lipoprotein that has been shown (by Western blot and Northern blot) to be expressed in the European B. burgdorferi strain B29, but not in the American strain B31. Cell envelope proteins of bacterial pathogens play important roles in the host-parasite interactions that occur during infection, including cell adherence, cell invasion, and immune cell activation or evasion. p27 is a basic protein of 248 amino acids with a typical prokaryotic leader sequence of 17 amino acid residues at the N-terminus of the proposed translation product. The p27 gene is located on a linear plasmid of a size of approximately 55 kb. Borrelia spirochetes are unique among diderm bacteria in their abundance of surface-displayed lipoproteins, some of which play important roles in the pathogenesis of Lyme disease and relapsing fever. There is evidence that Borrelia lipoproteins are specifically targeted to the bacterial surface, but that they can be retained in the periplasm by sequence-specific signals.

Gen-ID: 1194336

NCBI Accession: WP_010890395

UniProt: O50951