Streptavidin Antikörper

Produktnummer ABIN964708

Hersteller Produkt- Nr.: 200-401-095

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch Streptavidin in ELISA, WB, IHC und DB. Er zeigt eine Reaktivität gegenüber Streptomyces avidinii.

Kurzübersicht für Streptavidin Antikörper (ABIN964708)

Target: Streptavidin

Reaktivität: Streptomyces avidinii

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Streptavidin Antikörper ist unkonjugiert

Applikation: ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Dot Blot (DB)

Produktdetails anti-Streptavidin Antikörper

Hersteller Produkt- Nr.: 200-401-095

Hersteller: Rockland

Verwendungszweck: Streptavidin Antibody

Kreuzreaktivität (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Streptavidin [Streptomyces avidinii].

Produktmerkmale: Synonyms: rabbit anti-Streptavidin Antibody, rabbit anti Streptavidin

Aufreinigung: Anti-Streptavidin antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Sterilität: Sterile filtered

Immunogen:

Immunogen: Streptavidin [Streptomyces avidinii]

Immunogen Type: Native Protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry Dilution: 1:1,500 - 1:5,000

Application Note: Anti-Streptavidin antibody has been tested by ELISA and dot blot and is suitable for western blot, and immunohistochemistry, as well as other assays requiring lot-to-lot consistency.

Western Blot Dilution: 1:3,000 - 1:12,000

ELISA Dilution: 1:200,000

Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Details zu Streptavidin

Target: Streptavidin

Hintergrund: Background: Streptavidin antibody is a 60 kDa protein purified from the bacterium Streptomyces avidinii. Streptavidin homo-tetramers have an extraordinarily high affinity for biotin (also known as vitamin B7). With a dissociation constant (Kd) on the order of ~10-14 Mol/L,[1] the binding of biotin to streptavidin is one of the strongest non-covalent interactions known in nature. Streptavidin is used extensively in molecular biology and bio-nanotechnology due to the streptavidin-biotin complex's resistance to organic solvents, denaturants (e.g. guanidinium chloride), detergents (e.g. SDS, Triton), proteolytic enzymes, and extremes of temperature and pH . Anti-STREPTAVIDIN antibody is ideal for investigators involved in GFP and Epitope research.