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Biotin Ligase Tag (BLT) Antikörper

BLT Wirt: Kaninchen Polyclonal unconjugated
Produktnummer ABIN964590
  • Target
    Biotin Ligase Tag (BLT) (BLT) (Biotin Ligase Tag (BLT))
    Wirt
    Kaninchen
    Klonalität
    Polyklonal
    Sequenz
    GLNDIFEAQK IEWH
    Spezifität
    This antiserum detects the minimal peptide substrate (GLNDIFEAQKIEWH) for the E. coli biotin ligase tag (BLT of BirA. This peptide is also called the biotin acceptor peptide (BAP) of the biotin carboxyl carrier protein (BCCP) subunit of acetyl CoA carboxylase of E. coli. Western blot analysis of B. subtilis strains producing either (BAP-tagged) SPoIIIAH (XWB49) or (BAP-tagged) SpoIIQ (XWB46) showed strong and specific reactivity to the antisera. Minor background bands of unknown identity may be observed. The antisera will recognize the BAP/BLT tag irrespective of the whether the tag is biotinylated (data not shown).
    Produktmerkmale
    N/A
    Immunogen
    GLNDIFEAQKIEWH (minimal BirA substrate, biotin is added to the sequence at the lysine.)
    Immunogen Type: Peptide
  • Kommentare

    Gene Name: BirA

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Konzentration
    90 mg/mL
    Buffer
    0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Lagerung
    4 °C/-20 °C
    Informationen zur Lagerung
    Store vial at 4 °C prior to restoration. For extended storage aliquot contents and freeze at -20 °C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 °C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.
    Haltbarkeit
    12 months
  • Target
    Biotin Ligase Tag (BLT) (BLT) (Biotin Ligase Tag (BLT))
    Substanzklasse
    Tag
    Hintergrund
    N/A
    Synonyms: biotin ligase; biotin operon repressor protein; birA; biotin holoenzyme synthetase; biotin-[acetyl-CoA carboxylase] synthetase.
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