Biotin Antikörper

Produktnummer ABIN964580

Hersteller Produkt- Nr.: 600-401-098

Der Kaninchen Polyklonal Anti-Biotin-Antikörper wurde für WB, ELISA und IHC validiert. Er ist geeignet, Biotin in Proben von zu detektieren. Es ist 1 Publikation verfügbar.

Kurzübersicht für Biotin Antikörper (ABIN964580)

Target: Biotin

Reaktivität: Bitte anfragen

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Biotin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Produktdetails anti-Biotin Antikörper

Hersteller Produkt- Nr.: 600-401-098

Hersteller: Rockland

Verwendungszweck: Biotin Antibody

Kreuzreaktivität (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Biotin conjugated IgG and Biotin conjugated Bovine Serum Albumin.

Produktmerkmale: Synonyms: rabbit anti Biotin Antibody, rabbit anti-biotin Antibody

Aufreinigung: Biotin Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Biotin coupled to sepharose beads.

Sterilität: Sterile filtered

Immunogen:

Immunogen: Biotin conjugated to Keyhole Limpet Hemocyanin (KLH)

Immunogen Type: Other

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry Dilution: User Optimized

Application Note: Anti-Biotin Antibody has been tested by ELISA and is suitable for immunoprecipitation, immunodiffusion, conjugation and most immunological methods requiring lot-to-lot consistency, high titer and specificity.

Western Blot Dilution: 1:2,000 - 1:10,000

ELISA Dilution: 1:200,000

Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1.02 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: This product is stable for several weeks at 4° C as an undiluted liquid. Aliquot contents and freeze at -20° C or below for extended storage.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature. Dilute only prior to immediate use. 

Haltbarkeit: 12 months

Referenzen für anti-Biotin Antikörper (ABIN964580)

Goulielmaki, Ioannidou, Tsekrekou, Stratigi, Poutakidou, Gkirtzimanaki, Aivaliotis, Evangelou, Topalis, Altmüller, Gorgoulis, Chatzinikolaou, Garinis: "Tissue-infiltrating macrophages mediate an exosome-based metabolic reprogramming upon DNA damage." in: Nature communications, Vol. 11, Issue 1, pp. 42, (2020) (PubMed).

Details zu Biotin

Target: Biotin

Substanzklasse: Chemical

Hintergrund: Background: Biotin Antibody detects Biotin. Biotin is a water-soluble B-complex vitamin (vitamin B7). It is composed of a ureido (tetrahydroimidizalone) ring fused with a tetrahydrothiophene ring. A valeric acid substituent is attached to one of the carbon atoms of the tetrahydrothiophene ring. Biotin is a coenzyme for carboxylase enzymes, involved in the synthesis of fatty acids, isoleucine, and valine, and in gluconeogenesis. Biotin is necessary for cell growth, the production of fatty acids, and the metabolism of fats and amino acids. Anti-Biotin Antibody is ideal for investigators involved in Cell Signaling and Cell Biology research.