AHSA1 Antikörper

Produktnummer ABIN964557

Produktdetails anti-AHSA1 Antikörper

Target: AHSA1 (Activator of HSP90 ATPase Activity 1 (AHSA1))

Reaktivität: Human, Maus, Schimpanse

Wirt: Ratte

Klonalität: Monoklonal

Konjugat: Dieser AHSA1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Spezifität: This product is an IgG fraction antibody purified from tissue culture supernatant by Protein-G chromatography, followed by extensive dialysis against the buffer stated above. It is directed against human AHA1 protein.   A BLAST analysis was used to suggest cross-reactivity with AHA1 protein from mouse, human and chimpanzee based on 100% homology with the immunizing sequence.  Reactivity against homologues from other sources is not known.

Kreuzreaktivität: Human, Schimpanse

Produktmerkmale: Activator of Hsp90 ATPase (AHA1) stimulates the inherent ATPase cycle of Hsp90, which is essential for its chaperone activity in vivo. The activation and/or stability of many of the key regulatory and signaling proteins of the eukaryotic cell depend on their interaction with the Hsp90 molecular chaperone. Hsp90 is assisted and regulated by co-chaperones that participate in an ordered series both to assist client-protein recruitment or release and to modulate progress through the ATPase coupled chaperone cycle. Structural analysis and mutagenesis show that binding of the N-terminal domain of AHA1 to Hsp90 promotes a conformational switch in the middle-segment catalytic loop (aa 370-390) of Hsp90 that exposes the catalytic Arg380 and enables its interaction with ATP in the N-terminal nucleotide-binding domain of the chaperone. Recent studies show that AHA1 modulates Hsp90-dependent stability of the folding of the cystic fibrosis transmembrane conductance regulator (CFTR) in the endoplasmic reticulum (ER). Down-regulation of AHA1 rescues misfolding of CFTR in cystic fibrosis.

Aufreinigung: affinity purified

Sterilität: Sterile filtered

Immunogen: This Protein G purified monoclonal antibody was produced in rats by repeated immunizations with full length recombinant mouse AHA1 protein followed by hybridoma development.
Immunogen Type: RecombinantProtein

Klon: 25F2-D9

Isotyp: IgG2a kappa

Anwendungsinformationen

Applikationshinweise: This Protein-G purified antibody has been tested for use in ELISA, immunohistochemistry and western blotting.  Specific conditions for reactivity should be optimized by the end user.  Expect a band approximately 38-40 kDa in size corresponding to AHA1 protein by western blotting in the appropriate cell lysate or extract.

Kommentare:

Gene Name: Ahsa1

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1.0 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Konservierungsmittel: Without preservative

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store vial at 4 °C prior to restoration. For extended storage aliquot contents and freeze at -20 °C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 °C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.

Haltbarkeit: 12 months

Details zu AHSA1

Target: AHSA1 (Activator of HSP90 ATPase Activity 1 (AHSA1))

Andere Bezeichnung: AHA1 (AHSA1 Produkte)

Synonyme: ahsa1 antikoerper, MGC52884 antikoerper, MGC132147 antikoerper, MGC80312 antikoerper, AHSA1 antikoerper, MGC89589 antikoerper, wu:fb83b05 antikoerper, zgc:136821 antikoerper, zgc:56075 antikoerper, AHA1 antikoerper, C14orf3 antikoerper, p38 antikoerper, BC023857 antikoerper, AHA1, activator of heat shock 90kDa protein ATPase homolog 1 S homeolog antikoerper, activator of Hsp90 ATPase activity 1 antikoerper, AHA1, activator of heat shock 90kDa protein ATPase homolog 1 L homeolog antikoerper, activator of HSP90 ATPase activity 1 antikoerper, AHA1, activator of heat shock 90kDa protein ATPase homolog 1 antikoerper, AHA1, activator of heat shock protein ATPase homolog 1a antikoerper, AHA1, activator of heat shock 90kDa protein ATPase homolog 1 (yeast) antikoerper, AHA1, activator of heat shock protein ATPase 1 antikoerper, ahsa1.S antikoerper, AHSA1 antikoerper, ahsa1.L antikoerper, ahsa1 antikoerper, ahsa1a antikoerper, Ahsa1 antikoerper

Hintergrund: Activator of Hsp90 ATPase (AHA1) stimulates the inherent ATPase cycle of Hsp90, which is essential for its chaperone activity in vivo.  The activation and/or stability of many of the key regulatory and signaling proteins of the eukaryotic cell depend on their interaction with the Hsp90 molecular chaperone.  Hsp90 is assisted and regulated by co-chaperones that participate in an ordered series both to assist client-protein recruitment or release and to modulate progress through the ATPase coupled chaperone cycle.  Structural analysis and mutagenesis show that binding of the N-terminal domain of AHA1 to Hsp90 promotes a conformational switch in the middle-segment catalytic loop (aa 370–390) of Hsp90 that exposes the catalytic Arg380 and enables its interaction with ATP in the N-terminal nucleotide-binding domain of the chaperone.  Recent studies show that AHA1 modulates Hsp90-dependent stability of the folding of the cystic fibrosis transmembrane conductance regulator (CFTR) in the endoplasmic reticulum (ER).  Down-regulation of AHA1 rescues misfolding of CFTR in cystic fibrosis.
Synonyms: Aha1, Ahsa1 antibody, Activator of Hsp90 ATPase, Activator of 90 kDa heat shock protein ATPase homolog 1 antibody

Gen-ID: 217737

NCBI Accession: NP_666148

UniProt: Q8BK64