V5 Epitope Tag Antikörper

Produktnummer ABIN962847

Zitiert in 4+ Publikationen. Der Maus Monoklonal anti-V5 Epitope Tag Antikörper (Klon 4C12E11) (ABIN962847) detektiert spezifisch V5 Epitope Tag in WB, ELISA, IP, IF, FACS und ICC.

Kurzübersicht für V5 Epitope Tag Antikörper (ABIN962847)

Target: V5 Epitope Tag

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser V5 Epitope Tag Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunoprecipitation (IP), Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Klon: 4C12E11

Produktdetails anti-V5 Epitope Tag Antikörper

Sequenz: GKPIPNPLLG LDST

Spezifität: THE^TM V5 Tag Antibody, mAb, Mouse recognizes C-terminal, N-terminal, and internal V5 tagged fusion proteins.

Produktmerkmale: THE^TM V5 Tag Antibody, mAb, Mouse is produced from the hybridoma resulting from fusion of SP2/0-Ag14 myeloma and B-lymphocytes obtained from mouse immunized with V5 tag peptide conjugated to KLH.

Aufreinigung: Protein A affinity column

Immunogen: V5 tag peptide conjugated - KLH

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise: Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.1 µg/mL
Western blot: 0.2-1 µg/mL
Immunoprecipitation: 1-5 µg/mL
FACS: 1-3 µg for 1 x 10^6 cells
ICC/IF: 1-3 µg/mL
Fusion Partner Fusion of SP2/0-Ag14 myeloma and B-lymphocytes

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Reconstitute the lyophilized product with deionized water (or equivalent) to make antibody concentration of 0.5 mg/mL.

Buffer: PBS, pH 7.4, containing 0.02 % Sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handhabung: Avoid repeated freezing and thawing cycles.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below.

Referenzen für anti-V5 Epitope Tag Antikörper (ABIN962847)

Liew, Assmann, Templin, Raum, Lipson, Rajan, Qiang, Hu, Kawamori, Lindberg, Philipson, Sonenberg, Goldfine, Stoffers, Mirmira, Urano, Kulkarni: "Insulin regulates carboxypeptidase E by modulating translation initiation scaffolding protein eIF4G1 in pancreatic ? cells." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 111, Issue 22, pp. E2319-28, (2014) (PubMed).

Wang, Li, Felsovalyi, Chen, Cardozo, Krogsgaard: "Quantitative analysis of T cell receptor complex interaction sites using genetically encoded photo-cross-linkers." in: ACS chemical biology, Vol. 9, Issue 9, pp. 2165-72, (2014) (PubMed).

Kim, Li, Ahn, Song, Zhang: "Inositol Polyphosphate-5-Phosphatase F (INPP5F) inhibits STAT3 activity and suppresses gliomas tumorigenicity." in: Scientific reports, Vol. 4, pp. 7330, (2014) (PubMed).

Van Gorp, Van Breedam, Van Doorsselaere, Delputte, Nauwynck: "Identification of the CD163 protein domains involved in infection of the porcine reproductive and respiratory syndrome virus." in: Journal of virology, Vol. 84, Issue 6, pp. 3101-5, (2010) (PubMed).

Details zu V5 Epitope Tag

Target: V5 Epitope Tag

Andere Bezeichnung: V5 Tag

Substanzklasse: Tag

Hintergrund: The V5 epitope tag (GKPIPNPLLGLDST) is derived from a small epitope which presents on the P and V proteins of the paramyxovirus of simian virus 5 (SV5). V5 Tag Antibody is a useful tool in detection of recombinant proteins containing the V5 epitope.