CA2 Antikörper

Produktnummer ABIN96021

Hersteller Produkt- Nr.: 200-4157-0100

Dieses Anti-CA2-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von CA2 in WB und ELISA. Geeignet für Rind (Kuh).

Kurzübersicht für CA2 Antikörper (ABIN96021)

Target: CA2 (Carbonic Anhydrase II (CA2))

Reaktivität: Rind (Kuh)

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CA2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA

Produktdetails anti-CA2 Antikörper

Hersteller Produkt- Nr.: 200-4157-0100

Hersteller: Rockland

Verwendungszweck: Carbonic Anhydrase II Antibody

Kreuzreaktivität (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Carbonic Anhydrase II [Bovine Erythrocytes].

Produktmerkmale: Synonyms: rabbit anti-Carbonic Anhydrase II Antibody, Carbonate dehydratase II antibody, Carbonic anhydrase 2 antibody, Carbonic anhydrase B antibody, Carbonic anhydrase C antibody, Carbonic anhydrase II antibody, Carbonic dehydratase antibody, CAC, CA-II

Aufreinigung: Anti-Carbonic Anhydrase II Antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Immunogen:

Immunogen: Carbonic Anhydrase II [Bovine Erythrocytes]

Immunogen Type: Native Protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Application Note: Anti-Carbonic Anhydrase II Antibody has been tested by Western blot and immunohistochemistry. This product is suitable to be assayed against 1.0 μg of Carbonic Anhydrase II [Bovine Erythrocytes] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) [code #611-1302] and TMBE [code # TMBE-1000] as a substrate for 30 minutes at room temperature.

Western Blot Dilution: 1:2,000 - 1:10,000

ELISA Dilution: 1:20,000 - 1:100,000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Konzentration: 1 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Details zu CA2

Target: CA2 (Carbonic Anhydrase II (CA2))

Andere Bezeichnung: CA2

Hintergrund: Background: Carbonic Anhydrase II Antibody is specific for carbonic anhydrase II. Carbonic anhydrases (or carbonate dehydratases) are a family of metalloenzymes that catalyze the rapid interconversion of carbon dioxide and water to bicarbonate and Hydrogen (or vice versa). This reversible reaction occurs slowly in the absence of the carbonic anhydrase catalyst. The active site of most carbonic anhydrases contains a zinc ion cofactor. One of the functions of the enzyme in animals is to interconvert carbon dioxide and bicarbonate to maintain acid-base balance in blood and other tissues, and to help transport carbon dioxide out of tissues.

Gen-ID: 280740

NCBI Accession: NP_848667

UniProt: P00921