Biotin Antikörper (FITC)

Produktnummer ABIN95619

Hersteller Produkt- Nr.: 600-102-098

Dieses Anti-Biotin-Antikörper ist ein Ziege Polyklonal-Antikörper zur Detektion von Biotin in FACS, FM und DB. Geeignet für .

Kurzübersicht für Biotin Antikörper (FITC) (ABIN95619)

Target: Biotin

Reaktivität: Bitte anfragen

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser Biotin Antikörper ist konjugiert mit FITC

Applikation: Flow Cytometry (FACS), Fluorescence Microscopy (FM), Dot Blot (DB)

Produktdetails anti-Biotin Antikörper (FITC)

Hersteller Produkt- Nr.: 600-102-098

Hersteller: Rockland

Verwendungszweck: Biotin Antibody Fluorescein Conjugated

Kreuzreaktivität (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Goat Serum, Biotinylated IgG and Biotinylated Bovine Serum Albumin.

Produktmerkmale: Synonyms: goat anti-Biotin Antibody Fluorescein Conjugation, FITC conjugated goat anti-Biotin Antibody

Aufreinigung: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Biotin coupled to sepharose beads.

Immunogen:

Immunogen: Biotin conjugated to Keyhole Limpet Hemocyanin (KLH)

Immunogen Type: Native Protein

Isotyp: IgG

Labeling Ratio: 4.6

Anwendungsinformationen

Applikationshinweise:

Flow Cytometry Dilution: User Optimized

Application Note: Anti-Biotin Fluorescein Conjugated antibody has been tested by dot blot and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

IF Microscopy Dilution: 1:500-1:2,500

Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution:

Reconstitution Volume: 1.0 mL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Konzentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Haltbarkeit: 12 months

Details zu Biotin

Target: Biotin

Substanzklasse: Chemical

Hintergrund: Background: Biotin Antibody detects Biotin. Biotin is a water-soluble B-complex vitamin (vitamin B7). It is composed of a ureido (tetrahydroimidizalone) ring fused with a tetrahydrothiophene ring. A valeric acid substituent is attached to one of the carbon atoms of the tetrahydrothiophene ring. Biotin is a coenzyme for carboxylase enzymes, involved in the synthesis of fatty acids, isoleucine, and valine, and in gluconeogenesis. Biotin is necessary for cell growth, the production of fatty acids, and the metabolism of fats and amino acids. Anti-Biotin Fluorescein Conjugated Antibody is ideal for investigators involved in Cell Signaling and Cell Biology research.