Biotin Antikörper (Alkaline Phosphatase (AP))

Produktnummer ABIN95612

Hersteller Produkt- Nr.: 600-105-098

Der Ziege Polyklonal Anti-Biotin-Antikörper wurde für WB, ELISA und IHC validiert. Er ist geeignet, Biotin in Proben von zu detektieren.

Kurzübersicht für Biotin Antikörper (Alkaline Phosphatase (AP)) (ABIN95612)

Target: Biotin

Reaktivität: Bitte anfragen

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser Biotin Antikörper ist konjugiert mit Alkaline Phosphatase (AP)

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Produktdetails anti-Biotin Antikörper (Alkaline Phosphatase (AP))

Hersteller Produkt- Nr.: 600-105-098

Hersteller: Rockland

Verwendungszweck: Biotin Antibody Alkaline Phosphatase Conjugated

Kreuzreaktivität (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Goat Serum and Biotinylated IgG.

Produktmerkmale: Synonyms: goat anti-Biotin Antibody Alkaline Phosphatase Conjugation, Alk Phos conjugated goat anti-Biotin Antibody

Aufreinigung: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Biotin coupled to sepharose beads.

Sterilität: Sterile filtered

Immunogen:

Immunogen: Biotin conjugated to Keyhole Limpet Hemocyanin (KLH)

Immunogen Type: Other

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry Dilution: 1:200 - 1:1,000

Application Note: Anti-Biotin Alkaline Phosphatase conjugated antibody has been tested by ELISA an is suitable for immunoblotting (western or dot blot), ELISA and immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency.

Western Blot Dilution: 1:500 - 1:2,500

ELISA Dilution: 1:3,000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1.0 mg/mL

Buffer:

Buffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50 % (v/v) Glycerol, pH 8.0

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.1 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.

Haltbarkeit: 12 months

Details zu Biotin

Target: Biotin

Substanzklasse: Chemical

Hintergrund: Background: Biotin Antibody detects Biotin. Biotin is a water-soluble B-complex vitamin (vitamin B7). It is composed of a ureido (tetrahydroimidizalone) ring fused with a tetrahydrothiophene ring. A valeric acid substituent is attached to one of the carbon atoms of the tetrahydrothiophene ring. Biotin is a coenzyme for carboxylase enzymes, involved in the synthesis of fatty acids, isoleucine, and valine, and in gluconeogenesis. Biotin is necessary for cell growth, the production of fatty acids, and the metabolism of fats and amino acids. Anti-Biotin Alkaline Phosphatase Conjugated Antibody is ideal for investigators involved in Cell Signaling and Cell Biology research.