Bilirubin Oxidase Antikörper (Biotin)

Produktnummer ABIN95598

Hersteller Produkt- Nr.: 200-106-212-0100

Der Ziege Polyklonal Anti-Bilirubin Oxidase-Antikörper wurde für WB, IP und ELISA validiert. Er ist geeignet, Bilirubin Oxidase in Proben von Myrothecium verrucaria zu detektieren.

Kurzübersicht für Bilirubin Oxidase Antikörper (Biotin) (ABIN95598)

Target: Bilirubin Oxidase

Reaktivität: Myrothecium verrucaria

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser Bilirubin Oxidase Antikörper ist konjugiert mit Biotin

Applikation: Western Blotting (WB), Immunoprecipitation (IP), ELISA

Produktdetails anti-Bilirubin Oxidase Antikörper (Biotin)

Hersteller Produkt- Nr.: 200-106-212-0100

Hersteller: Rockland

Verwendungszweck: Bilirubin Oxidase Antibody Biotin Conjugated

Kreuzreaktivität (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum as well as purified and partially purified Bilirubin Oxidase [Myrothecium verrucaria].

Produktmerkmale: Synonyms: goat anti-Bilirubin Oxidase biotin conjugation, goat anti-Bilirubin Oxidase Antibody biotin Conjugated

Aufreinigung: Anti-Bilirubin Oxidase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Immunogen:

Immunogen: Bilirubin Oxidase [Myrothecium verrucaria]

Immunogen Type: Native Protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Application Note: Anti-Bilirubin Oxidase has been tested by western blot and is suitable to be assayed against 1.0 μg of Bilirubin Oxidase in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:1,000 to 1:3,000 of the reconstitution concentration is suggested for this product.

Western Blot Dilution: 1:500 - 1:2,500

Immunoprecipitation Dilution: 1:100

ELISA Dilution: 1:1,000 - 1:5,000

Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Konzentration: 10.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Haltbarkeit: 12 months

Details zu Bilirubin Oxidase

Target: Bilirubin Oxidase

Hintergrund: Background: Anti-Bilirubin Oxidase antibody is an enzyme that increases the oxidation rate of bilirubin to biliverdin and other substrates. It is ideal for investigators interested in Metabolism or Signal Transduction research.

UniProt: Q12737