Telefon:
+49 (0)241 95 163 153
Fax:
+49 (0)241 95 163 155
E-Mail:
orders@antikoerper-online.de

PF4 Antikörper (Biotin)

Der Maus Monoklonal Anti-PF4-Antikörper wurde für WB und EIA validiert. Er ist geeignet, PF4 in Proben von Human zu detektieren.
Produktnummer ABIN954176

Kurzübersicht für PF4 Antikörper (Biotin) (ABIN954176)

Target

Alle PF4 Antikörper anzeigen
PF4 (Platelet Factor 4 (PF4))

Reaktivität

  • 83
  • 21
  • 17
  • 3
  • 2
  • 2
  • 2
  • 1
  • 1
Human

Wirt

  • 88
  • 31
  • 5
  • 1
  • 1
  • 1
Maus

Klonalität

  • 93
  • 34
Monoklonal

Konjugat

  • 75
  • 18
  • 15
  • 4
  • 4
  • 3
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Dieser PF4 Antikörper ist konjugiert mit Biotin

Applikation

  • 102
  • 73
  • 48
  • 39
  • 33
  • 12
  • 9
  • 7
  • 7
  • 7
  • 6
  • 4
  • 3
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Western Blotting (WB), Enzyme Immunoassay (EIA)

Klon

KKO
  • Spezifität

    This antibody is specific for Human Platelet Factor IV/heparin complexes. AM31851BT-N (clone KKO), unlike AM31850BT-N (clone RTO) which binds PF4 alone, is specific for PF4/heparin complexes and is seen to have similar binding properties as auto-antibodies found in HIT/HITT.

    Kreuzreaktivität (Details)

    Species reactivity (tested):Human.

    Immunogen

    Human Factor IV purified from the supernatant of Thrombin activated platelets.

    Isotyp

    IgG2b
  • Applikationshinweise

    ELISA: Use at 0.08 μg/mL following coating plate with PF4/ heparin complex. This can be done by incubating PF4 with an excess of heparin (100-200 U/mL), while coating the plate.

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Konzentration

    0.1 mg/mL

    Buffer

    PBS containing 0.02 % Sodium Azide as preservative and EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/mL

    Konservierungsmittel

    Sodium azide

    Vorsichtsmaßnahmen

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Handhabung

    Avoid repeated freezing and thawing.

    Lagerung

    4 °C/-20 °C

    Informationen zur Lagerung

    Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
  • Target

    PF4 (Platelet Factor 4 (PF4))

    Andere Bezeichnung

    Platelet Factor 4 / PF4

    Hintergrund

    Platelet Factor IV (PF4) is a 70 aa protein released from the alpha granules of activated platelets. PF4 is synthesized by bone marrow megakaryocytes and stored in alpha granules as a non-covalent bound tetramer. Platelet factor IV binds with high affinity to heparin and plays a role in inflammation and wound repair. PF4 is a chemoattractant for neutrophils, monocytes and fibroblasts and has been reported to be an immunologic regulator that inhibits suppressor T-cell activity. Heparin-Induced Thrombocytopenia/Thrombosis (HIT/HITT) is a life-threatening complication that manifests itself in a small population of patients exposed to intravenous-heparin. It is characterized by the production of PF4/heparin auto-antibodies. These auto-antibodies bind the PF4/heparin complexes and subsequently bind the FCGIIa receptor on the platelets surface through their Fc region. This activates the platelets and can initiate clot formation.Synonyms: C-X-C motif chemokine 4, CXCL4, Iroplact, Oncostatin-A, PF-4, SCYB4

    Gen-ID

    5196

    NCBI Accession

    NP_002610

    UniProt

    P02776
Sie sind hier:
Chat with us!