PF4 Antikörper (Biotin)

Produktnummer ABIN954176

Der Maus Monoklonal anti-PF4 Antikörper wird verwendet zum Nachweis von PF4 in Proben von Human. Er wurde validiert für WB und EIA.

Kurzübersicht für PF4 Antikörper (Biotin) (ABIN954176)

Target: PF4 (Platelet Factor 4 (PF4))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser PF4 Antikörper ist konjugiert mit Biotin

Applikation: Western Blotting (WB), Enzyme Immunoassay (EIA)

Klon: KKO

Produktdetails anti-PF4 Antikörper (Biotin)

Spezifität: This antibody is specific for Human Platelet Factor IV/heparin complexes. AM31851BT-N (clone KKO), unlike AM31850BT-N (clone RTO) which binds PF4 alone, is specific for PF4/heparin complexes and is seen to have similar binding properties as auto-antibodies found in HIT/HITT.

Kreuzreaktivität (Details): Species reactivity (tested):Human.

Immunogen: Human Factor IV purified from the supernatant of Thrombin activated platelets.

Isotyp: IgG2b

Anwendungsinformationen

Applikationshinweise: ELISA: Use at 0.08 μg/mL following coating plate with PF4/ heparin complex. This can be done by incubating PF4 with an excess of heparin (100-200 U/mL), while coating the plate.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.1 mg/mL

Buffer: PBS containing 0.02 % Sodium Azide as preservative and EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/mL

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu PF4

Target: PF4 (Platelet Factor 4 (PF4))

Andere Bezeichnung: Platelet Factor 4 / PF4

Hintergrund: Platelet Factor IV (PF4) is a 70 aa protein released from the alpha granules of activated platelets. PF4 is synthesized by bone marrow megakaryocytes and stored in alpha granules as a non-covalent bound tetramer. Platelet factor IV binds with high affinity to heparin and plays a role in inflammation and wound repair. PF4 is a chemoattractant for neutrophils, monocytes and fibroblasts and has been reported to be an immunologic regulator that inhibits suppressor T-cell activity. Heparin-Induced Thrombocytopenia/Thrombosis (HIT/HITT) is a life-threatening complication that manifests itself in a small population of patients exposed to intravenous-heparin. It is characterized by the production of PF4/heparin auto-antibodies. These auto-antibodies bind the PF4/heparin complexes and subsequently bind the FCGIIa receptor on the platelets surface through their Fc region. This activates the platelets and can initiate clot formation.Synonyms: C-X-C motif chemokine 4, CXCL4, Iroplact, Oncostatin-A, PF-4, SCYB4

Gen-ID: 5196

NCBI Accession: NP_002610

UniProt: P02776