Nestin Antikörper

Produktnummer ABIN953652

Dieses Anti-Nestin-Antikörper ist ein Huhn Polyklonal-Antikörper zur Detektion von Nestin in WB, IF und IHC (p). Geeignet für Maus und Ratte.

Kurzübersicht für Nestin Antikörper (ABIN953652)

Target: Nestin (NES)

Reaktivität: Maus, Ratte

Wirt: Huhn

Klonalität: Polyklonal

Konjugat: Dieser Nestin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-Nestin Antikörper

Spezifität: Recognizes Nestin. Note that the sequences of the Mouse and Human Nestin genes are divergent, and it is unlikely that antibodies raised against the Mouse sequence would recognize Human Nestin.

Aufreinigung: Affinity Chromatography using a peptide column.

Immunogen: Three synthetic peptides KLH conjugated corresponding to different regions of the Mouse Nestin gene product (NP_057910), but are shared with the Rat (NM_012987) protein sequence. Production: After repeated injections, immune eggs were collected, and the IgY fractions were purified from the yolks. These IgY fractions were then affinity-purified using a peptide column, and the concentrations of the eluates adjusted to 0.3 mg/ml. Finally, equal volumes of each of the three affinity-purified anti-peptide antibodies were mixed, and the preparation was filter-sterilized.

Isotyp: Ig Fraction

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.3 mg/mL

Buffer: 10 mM PBS, pH 7.2 containing 0.02 % Sodium Azide as preservative.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Store the antibody undiluted in the dark at 2-8 °C.

Details zu Nestin

Target: Nestin (NES)

Andere Bezeichnung: Nestin

Hintergrund: Mouse Nestin is a 206,994 dalton protein (1864 amino acids) expressed in neural crest cells, CNS neural stems cells, as well as a few non-neural cell types in the embryo, including cells within the pancreatic islets of Langerhans and the limb bud. Nestin is an intermediate filament-associated protein, and contributes to the cytoskeleton.Synonyms: NES, Nbla00170

Gen-ID: 18008

NCBI Accession: NP_057910