ACPP Antikörper

Produktnummer ABIN950249

Der Huhn Polyklonal anti-ACPP Antikörper wird verwendet zum Nachweis von ACPP in Proben von Maus. Er wurde validiert für IF und IHC (p).

Kurzübersicht für ACPP Antikörper (ABIN950249)

Target: ACPP (Acid Phosphatase, Prostate (ACPP))

Reaktivität: Maus

Wirt: Huhn

Klonalität: Polyklonal

Konjugat: Dieser ACPP Antikörper ist unkonjugiert

Applikation: Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-ACPP Antikörper

Spezifität: Recognizes Mouse Prostatic Acid Phosphatase (PAP).

Aufreinigung: Affinity Chromatography using a peptide column.

Immunogen: Recombinant Mouse PAP protein was expressed using a baculoviral-delivery system. Preparation: After repeated injections, immune eggs were collected from laying hens, from which IgY antibody were prepared (“anti-PAP IgY fraction”). Some of this antibody was further purified using an agarose matrix to which the PAP protein was convalently attached (“Affinity-purified anti-PAP”). The final preparation in the accompanying vial contains 10 mg/ml of the “anti-PAP IgY fraction” supplemented with 20 mg/ml of the “affinity-purified anti-PAP” plus 50% (v/v) Glycerol (to prevent freezing at –20°C). Finally, this antibody preparation was filter-sterilized (0.45 mm) and 200 µl aliquots prepared.

Isotyp: Ig Fraction

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 10 mg IgY/ml (based on absorbance at 280 nm)

Buffer: 10 mM PBS, pH 7.2 containing 1 % BSA as stabilizer and 0.02 % Sodium Azide as preservative.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Store the antibody undiluted in the dark at 2-8 °C.

Details zu ACPP

Target: ACPP (Acid Phosphatase, Prostate (ACPP))

Andere Bezeichnung: ACPP / Prostatic Acid Phosphatase

Hintergrund: Mouse PAP is a 43,698 dalton protein (381 amino acids, NCBI accession numberAAF23171) associated with prostatic cancer cells, as well as primary afferent sensory neurons involved in the pain pathway. This protein is an enzyme that dephosphorylates adenosine monophosphate (AMP) in the dorsal horn gray matter of the spinal cord, generating free adenosine. Injections of PAP into the dorsal horn of experimental mice has been shown to decrease pain perception by acting in an antinociceptive, antihyperalgesic, and antiallodynic fashion.Synonyms: ACP3, PAP, PSAP, Prostate acid phosphatase

Gen-ID: 56318

NCBI Accession: NP_062781

Pathways: Synaptic Membrane, Ribonucleoside Biosynthetic Process