Alkaline Phosphatase Antikörper

Produktnummer ABIN94942

Hersteller Produkt- Nr.: 100-4134

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch Alkaline Phosphatase in WB und ELISA. Er zeigt eine Reaktivität gegenüber E. coli und wurde in 2+ Publikationen erwähnt.

Kurzübersicht für Alkaline Phosphatase Antikörper (ABIN94942)

Target: Alkaline Phosphatase (ALP)

Reaktivität: E. coli

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Alkaline Phosphatase Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA

Produktdetails anti-Alkaline Phosphatase Antikörper

Hersteller Produkt- Nr.: 100-4134

Hersteller: Rockland

Verwendungszweck: Alkaline Phosphatase Antibody

Produktmerkmale: Synonyms: rabbit anti-Alkaline Phosphatase Antibody, Akp2 antibody, ALP antibody, Nagao isozyme antibody, Placental alkaline phosphatase antibody, Placental like alkaline phosphatase antibody, PLAP 1 antibody, PLAP like antibody, Regan isozyme antibody, Kasahara isozyme antibody

Aufreinigung: Anti-Alkaline Phosphatase (E.Coli) Antibody was prepared from monospecific antiserum by a delipidation and defibrination.

Immunogen:

Immunogen: Alkaline Phosphatase [E.coli]

Immunogen Type: Native Protein

Anwendungsinformationen

Applikationshinweise:

Application Note: Anti-Alkaline Phosphatase (E. Coli) Antibody has been tested against 1.0 μg of Alkaline Phosphatase [E.coli] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) code #611-1302 and (ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:20,000 to 1:100,000 of the reconstitution concentration is suggested for Anti-Alkaline Phosphatase (E. Coli) Antibody.

Western Blot Dilution: 1:500 - 1:2,000

ELISA Dilution: 1:8,000 - 1:40,000

Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution:

Reconstitution Volume: 2.0 mL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Konzentration: 75 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Haltbarkeit: 12 months

Referenzen für anti-Alkaline Phosphatase Antikörper (ABIN94942)

Epstein, Kiechle, Artiss, Zak: "The clinical use of alkaline phosphatase enzymes." in: Clinics in laboratory medicine, Vol. 6, Issue 3, pp. 491-505, (1986) (PubMed).

Lange, Millan, Stigbrand, Vessella, Ruoslahti, Fishman: "Placental alkaline phosphatase as a tumor marker for seminoma." in: Cancer research, Vol. 42, Issue 8, pp. 3244-7, (1982) (PubMed).

Details zu Alkaline Phosphatase

Target: Alkaline Phosphatase (ALP)

Andere Bezeichnung: Alkaline Phosphatase

Hintergrund: Background: Alkaline Phosphatase is an enzyme that is key in the hydrolysis of many different types of molecules, resulting in the removal of phosphate groups from said molecule. Most active in basic environments and a common serum analyte, alkaline phosphatase and its isoenzymes are good indicators of bone, liver, and testicular diseases/cancers in immunoassays and diagnostics. Anti-Alkaline Phosphatase (E. Coli) Antibody is ideal for investigators in Cancer, Immunology, and Molecular Biology.

Gen-ID: 945041

NCBI Accession: NP_414917

UniProt: P00634