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ALDOA Antikörper

Dieses Goat Polyclonal-Antikörper erkennt spezifisch ALDOA in WB, ELISA, IP. Er zeigt eine Reaktivität gegenüber Human, Rabbit und wurde in 1 Publikation erwähnt.
Rockland
Produktnummer ABIN94900
Hersteller Produkt- Nr.: 100-1141

Kurzübersicht für ALDOA Antikörper (ABIN94900)

Target

Alle ALDOA Antikörper anzeigen
ALDOA (Aldolase A, Fructose-Bisphosphate (ALDOA))

Reaktivität

  • 86
  • 33
  • 31
  • 16
  • 5
  • 2
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
Human, Kaninchen

Wirt

  • 65
  • 16
  • 11
  • 2
Ziege

Klonalität

  • 74
  • 19
Polyklonal

Konjugat

  • 65
  • 7
  • 6
  • 3
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 1
Dieser ALDOA Antikörper ist unkonjugiert

Applikation

  • 75
  • 43
  • 32
  • 23
  • 21
  • 15
  • 10
  • 8
  • 7
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
Western Blotting (WB), ELISA, Immunoprecipitation (IP)
  • Hersteller Produkt- Nr.

    100-1141

    Hersteller

    Rockland

    Verwendungszweck

    Aldolase Antibody

    Kreuzreaktivität (Details)

    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-goat serum, purified and partially purified Aldolase [Rabbit Muscle].

    Produktmerkmale

    Synonyms: goat anti-Aldolase Antibody, Fructose-bisphosphate aldolase A, Muscle-type aldolase

    Aufreinigung

    This product was prepared from monospecific antiserum by a delipidation and defibrination.

    Immunogen

    Immunogen: Aldolase [Rabbit Muscle]

    Immunogen Type: Native Protein

  • Applikationshinweise

    Application Note: Anti-Aldolase has been tested by immunoprecipitation and western blot and is suitable to be assayed against 1.0 μg of Aldolase [Rabbit Muscle] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Goat IgG [H&L] (Rabbit) code #605-4302 and (ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:3,000 to 1:12,000 of the reconstitution concentration is suggested for this product.  Use approximately 5 µL of antibody to immunoprecipitate 50 µL of protein lysate.

    Western Blot Dilution: 1:500 - 1:5,000

    Immunoprecipitation Dilution: 1:100

    ELISA Dilution: 1:5,000 - 1:20,000

    Other: User Optimized

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Format

    Lyophilized

    Rekonstitution

    Reconstitution Volume: 2.0 mL

    Reconstitution Buffer: Restore with deionized water (or equivalent)

    Konzentration

    90 mg/mL

    Buffer

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: None

    Preservative: 0.01 % (w/v) Sodium Azide

    Konservierungsmittel

    Sodium azide

    Vorsichtsmaßnahmen

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Lagerung

    4 °C,-20 °C

    Informationen zur Lagerung

    Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

    Haltbarkeit

    12 months
  • Shitashige, Naishiro, Idogawa, Honda, Ono, Hirohashi, Yamada: "Involvement of splicing factor-1 in beta-catenin/T-cell factor-4-mediated gene transactivation and pre-mRNA splicing." in: Gastroenterology, Vol. 132, Issue 3, pp. 1039-54, (2007) (PubMed).

  • Target

    ALDOA (Aldolase A, Fructose-Bisphosphate (ALDOA))

    Andere Bezeichnung

    ALDOA

    Hintergrund

    Background: Aldolase plays a key role in glycolysis and gluconeogenesis. In addition, it may also function as scaffolding protein. In vertebrates, three forms of this ubiquitous glycolytic enzyme are found, aldolase A in muscle, aldolase B in the liver, and aldolase C in the brain. Alkylation of Arg-43 inactivates the enzyme. Aldolase is involved in step 4 of the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose.

    Gen-ID

    100009055

    NCBI Accession

    NP_001075707

    UniProt

    P00883

    Pathways

    Ribonucleoside Biosynthetic Process
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