ALT Antikörper

Produktnummer ABIN94798

Hersteller Produkt- Nr.: 100-601-206

Dieses Schaf Polyklonal-Antikörper erkennt spezifisch ALT in WB und ELISA. Er zeigt eine Reaktivität gegenüber Schwein.

Kurzübersicht für ALT Antikörper (ABIN94798)

Target: ALT (Alanine Aminotransferase (ALT))

Reaktivität: Schwein

Wirt: Schaf

Klonalität: Polyklonal

Konjugat: Dieser ALT Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA

Produktdetails anti-ALT Antikörper

Hersteller Produkt- Nr.: 100-601-206

Hersteller: Rockland

Verwendungszweck: Alanine Transaminase Antibody

Kreuzreaktivität (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-sheep serum, purified and partially purified Alanine Transaminase (ALT) [Pig Heart].

Produktmerkmale: Synonyms: sheep anti-Alanine Transaminase Antibody, AAT1 antibody, Alanine aminotransferase 1 antibody, alanine aminotransferase antibody, ALT 1 antibody, ALT1 antibody, Glutamic alanine transaminase 1 antibody

Aufreinigung: This product was prepared from monospecific antiserum by a delipidation and defibrination.

Immunogen:

Immunogen: Alanine Transaminase (ALT) [Pig Heart]

Immunogen Type: Native Protein

Anwendungsinformationen

Applikationshinweise:

Application Note: Anti-Alanine Transaminase has been assayed against 1.0 μg of Alanine Transaminase (ALT) [Pig Heart] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Sheep IgG [H&L] (Goat) code #611-1302 and (ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:1,000 to 1:5,000 of the reconstitution concentration is suggested for this product.

Western Blot Dilution: 1:500 - 1:3,000

ELISA Dilution: 1:5,000 - 1:25,000

Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution:

Reconstitution Volume: 2.0 mL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Konzentration: 96 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Details zu ALT

Target: ALT (Alanine Aminotransferase (ALT))

Andere Bezeichnung: GPT

Hintergrund: Background: Alanine Transaminase catalyzes the reversible transamination between alanine and 2-oxoglutarate to form pyruvate and glutamate. It participates in cellular nitrogen metabolism and also in liver gluconeogenesis starting with precursors transported from skeletal muscles.

Gen-ID: 2875, 113565

UniProt: P13191