ATP1A1 Antikörper

Produktnummer ABIN94710

Hersteller Produkt- Nr.: 200-9155

Dieses Anti-ATP1A1-Antikörper ist ein Huhn Polyklonal-Antikörper zur Detektion von ATP1A1 in WB, ELISA und IP. Geeignet für Hund.

Kurzübersicht für ATP1A1 Antikörper (ABIN94710)

Target: ATP1A1 (Sodium Potassium ATPase, alpha1 (ATP1A1))

Reaktivität: Hund

Wirt: Huhn

Klonalität: Polyklonal

Konjugat: Dieser ATP1A1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Produktdetails anti-ATP1A1 Antikörper

Hersteller Produkt- Nr.: 200-9155

Hersteller: Rockland

Verwendungszweck: ATPase Antibody

Kreuzreaktivität (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Chicken Serum as well as purified and partially purified ATPase, Sodium, Potassium [Canine Kidney].

Produktmerkmale: Synonyms: chicken anti-ATPase Antibody, Sodium/potassium-transporting ATPase

Aufreinigung: This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Immunogen:

Immunogen: ATPase, Sodium, Potassium [Canine Kidney]

Immunogen Type: Native Protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Application Note: Anti-ATPase Na+ K+ has been assayed against 1.0 μg of ATPase, Sodium, Potassium in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Chicken IgG [H&L] (Rabbit) code #603-4302 and (ABTS (2,2'-azino-bis-[3-ethylbenthia-zoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:1,000 to 1:6,000 of the reconstitution concentration is suggested for this product.

Western Blot Dilution: 1:500 - 1:5,000

Immunoprecipitation Dilution: 1:100

ELISA Dilution: 1:5,000 - 1:20,000

Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution:

Reconstitution Volume: 1.0 mL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Konzentration: 10.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Haltbarkeit: 12 months

Details zu ATP1A1

Target: ATP1A1 (Sodium Potassium ATPase, alpha1 (ATP1A1))

Andere Bezeichnung: ATP1A1

Hintergrund: Background: Sodium/potassium-transporting ATPase subunit alpha-1 is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients.

Gen-ID: 403992

NCBI Accession: NP_001003306

UniProt: P50997

Pathways: Thyroid Hormone Synthesis, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Proton Transport, Ribonucleoside Biosynthetic Process