ADA Antikörper

Produktnummer ABIN94705

Hersteller Produkt- Nr.: 100-401-140

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch ADA in WB, ELISA und IP. Er zeigt eine Reaktivität gegenüber Human, Maus und Rind (Kuh).

Kurzübersicht für ADA Antikörper (ABIN94705)

Target: ADA (Adenosine Deaminase (ADA))

Reaktivität: Human, Maus, Rind (Kuh)

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser ADA Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Produktdetails anti-ADA Antikörper

Hersteller Produkt- Nr.: 100-401-140

Hersteller: Rockland

Verwendungszweck: Adenosine Deaminase Antibody

Kreuzreaktivität (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-rabbit serum, purified and partially purified Adenosine Deaminase [Calf Spleen].

Produktmerkmale: Synonyms: rabbit anti-Adenosine Deaminase Antibody, Adenosine deaminase, Adenosine aminohydrolase

Aufreinigung: This product was prepared from monospecific antiserum by a delipidation and defibrination.

Immunogen:

Immunogen: Adenosine Deaminase [Calf Spleen]

Immunogen Type: Native Protein

Anwendungsinformationen

Applikationshinweise:

Application Note: Anti-Adenosine Deaminase has been tested by western blot and is suitable for use in ELISA, western blot, and immunoprecipitation. Specific conditions for reactivity should be optimized by the end user.

Western Blot Dilution: 1:500 - 1:2,000

Immunoprecipitation Dilution: 1:100

ELISA Dilution: 1:5,000 - 1:10,000

Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution:

Reconstitution Volume: 2.0 mL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Konzentration: 90 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Haltbarkeit: 12 months

Details zu ADA

Target: ADA (Adenosine Deaminase (ADA))

Andere Bezeichnung: ADA

Hintergrund: Background: Adenosine Deaminase catalyzes the hydrolytic deamination of adenosine and 2-deoxyadenosine. It plays an important role in purine metabolism and in adenosine homeostasis. Adenosine Deaminase modulates signaling by extracellular adenosine, therefore contributes indirectly to cellular signaling events. It acts as a positive regulator of T-cell coactivation, by binding DPP4. Its interaction with DPP4 regulates lymphocyte-epithelial cell adhesion. Adenosine Deaminase enhances dendritic cell immunogenicity by affecting dendritic cell costimulatory molecule expression and cytokines and chemokines secretion. It enhances CD4+ T-cell differentiation and proliferation. And acts as a positive modulator of adenosine receptors ADORA1 and ADORA2A, by enhancing their ligand affinity via conformational change. It stimulates plasminogen activation. It also plays a role in male fertility and a protective role in early post implantation embryonic development.

Gen-ID: 280712

NCBI Accession: NP_776312

UniProt: P56658

Pathways: Regulation of G-Protein Coupled Receptor Protein Signaling, Ribonucleoside Biosynthetic Process