Dieser Acid Phosphatase Antikörper ist unkonjugiert
Applikation
Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
Kreuzreaktivität (Details)
Cross reactivity against Acid Phosphatase from other tissues and species may occur but have not been specifically determined.
Reinheit
Anti-ACID PHOSPHATASE is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Acid Phosphatase [Potato].
Endotoxin-Niveau
Low Endotoxin : No
Immunogen
Acid Phosphatase [Potato] Immunogen Type: Native Protein
ACP
Reaktivität: Maus
WB, IF (cc), IF (p)
Wirt: Kaninchen
Polyclonal
Alexa Fluor 555
Applikationshinweise
ELISA Dilution: 1:4.000 - 1:40.000 Immunohistochemistry Dilution: 1:1.000 - 1:5.000 Western Blot Dilution: 1:2.000 - 1:10.000
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Liquid
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Handhabung
Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
Lagerung
-20 °C
Informationen zur Lagerung
Store vial at -20 °C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below.
Haltbarkeit
Expiration date is one (1) year from date of opening.