Acetylcholinesterase Antikörper (Biotin)

Produktnummer ABIN94540

Hersteller Produkt- Nr.: 200-106-232-0100

Dieses Anti-Acetylcholinesterase-Antikörper ist ein Ziege Polyklonal-Antikörper zur Detektion von Acetylcholinesterase in WB, ELISA und IP. Geeignet für Rind (Kuh).

Kurzübersicht für Acetylcholinesterase Antikörper (Biotin) (ABIN94540)

Target: Acetylcholinesterase (AChE)

Reaktivität: Rind (Kuh)

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser Acetylcholinesterase Antikörper ist konjugiert mit Biotin

Applikation: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Produktdetails anti-Acetylcholinesterase Antikörper (Biotin)

Hersteller Produkt- Nr.: 200-106-232-0100

Hersteller: Rockland

Verwendungszweck: Acetyl Cholinesterase Antibody Biotin Conjugated

Kreuzreaktivität (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum as well as purified and partially purified Acetyl Cholinesterase [Bovine Erythrocytes].

Produktmerkmale: Synonyms: goat anti-Acetyl Cholinesterase Antibody, biotin conjugated goat anti-Acetyl Cholinesterase Antibody, Acetylcholinesterase, AChE

Aufreinigung: Acetyl Cholinesterase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Immunogen:

Immunogen: Acetyl Cholinesterase [Bovine Erythrocytes]

Immunogen Type: Native Protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Application Note: Anti-Acetyl Cholinesterase Biotin Conjugated Antibody has been tested by western blot and is suitable to be assayed against 1.0 μg of Acetyl Cholinesterase in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:1,000 to 1:2,000 is suggested for this product.

Western Blot Dilution: 1:500 - 1:2,500

Immunoprecipitation Dilution: 1:100

ELISA Dilution: 1:2,000 - 1:10,000

Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Konzentration: 10 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Haltbarkeit: 12 months

Details zu Acetylcholinesterase

Target: Acetylcholinesterase (AChE)

Andere Bezeichnung: ACHE

Hintergrund: Background: Acetyl Cholinesterase is abundantly expressed in erythrocytes and is part of the type-B carboxylesterase/lipase family. Anti-Acetyl Cholinesterase cancels signal transduction at the neuromuscular junction via hydrolysis of acetylcholine as it is released into the synaptic cleft by the motor neurons. Anti-Acetyl Cholinesterase antibody is ideal for investigators involved in Neuroscience and Neurotransmitter research.

Gen-ID: 540446

NCBI Accession: NP_001069688

UniProt: P23795

Pathways: Skeletal Muscle Fiber Development