Acetylcholinesterase Antikörper
Kurzübersicht für Acetylcholinesterase Antikörper (ABIN94539)
Target
Alle Acetylcholinesterase (AChE) Antikörper anzeigenReaktivität
Wirt
Klonalität
Konjugat
Applikation
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Hersteller Produkt- Nr.
- 100-101-232
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Hersteller
- Rockland
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Verwendungszweck
- Acetyl Cholinesterase Antibody
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Kreuzreaktivität (Details)
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-goat serum, purified and partially purified Acetyl Cholinesterase [Bovine Erythrocytes].
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Produktmerkmale
- Synonyms: goat anti-Acetyl Cholinesterase Antibody, Acetyl cholinesterase, AChE, Acetylcholinesterase
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Aufreinigung
- Anti-Acetyl Cholinesterase was prepared from monospecific antiserum by a delipidation and defibrination.
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Immunogen
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Immunogen: Acetyl Cholinesterase [Bovine Erythrocytes]
Immunogen Type: Native Protein
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Applikationshinweise
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Application Note: This product has been assayed against 1.0 μg of Acetyl Cholinesterase [Bovine Erythrocytes] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Goat IgG [H&L] (Goat) code #611-1302 and (ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:20,000 to 1:100,000 of the reconstitution concentration is suggested for this product.
Western Blot Dilution: 1:500 - 1:5,000
Immunoprecipitation Dilution: 1:100
ELISA Dilution: 1:5,000 - 1:20,000
Other: User Optimized
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Beschränkungen
- Nur für Forschungszwecke einsetzbar
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Format
- Lyophilized
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Rekonstitution
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Reconstitution Volume: 2.0 mL
Reconstitution Buffer: Restore with deionized water (or equivalent)
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Konzentration
- 90 mg/mL
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Buffer
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide -
Konservierungsmittel
- Sodium azide
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Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Lagerung
- 4 °C,-20 °C
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Informationen zur Lagerung
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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Haltbarkeit
- 12 months
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- Acetylcholinesterase (AChE)
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Andere Bezeichnung
- ACHE
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Hintergrund
- Background: Acetylcholinesterase terminates signal transduction at the neuromuscular junction by rapid hydrolysis of the acetylcholine released into the synaptic cleft.
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Gen-ID
- 540446
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NCBI Accession
- NP_001069688
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UniProt
- P23795
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Pathways
- Skeletal Muscle Fiber Development
Target
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