NEFM Antikörper

Produktnummer ABIN94435

Dieses Maus Monoklonal-Antikörper erkennt spezifisch NEFM in WB, ICC und IHC (p). Er zeigt eine Reaktivität gegenüber Säugetier und wurde in 1 Publikation erwähnt.

Kurzübersicht für NEFM Antikörper (ABIN94435)

Target: NEFM (Neurofilament Triplet M Protein (NEFM))

Reaktivität: Säugetier

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser NEFM Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Klon: NF-09

Produktdetails anti-NEFM Antikörper

Verwendungszweck: Anti-Neurofilament M Purified

Spezifität: The antibody NF-09 reacts with both phosphorylated and non-phosphorylated form of medium neurofilament protein (160 kDa intracellular antigen) of various species.

Kreuzreaktivität (Details): Mammalian

Aufreinigung: Purified by protein-A affinity chromatography.

Reinheit: > 95 % (by SDS-PAGE)

Immunogen: Pellet of porcine brain cold stable proteins after depolymerization of microtubules.

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise: Immunocytochemistry: Positive tissue: Neuro2A murine cell line, Carnoys fixative 2 x 3 min, blocking 1 % glycine + 0.2 % gelatin 10 min.
Western blotting: Recommended dilution: 1-2 μg/mL.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Do not freeze.

Lagerung: 4 °C

Informationen zur Lagerung: Store at 2-8°C. Do not freeze.

Referenzen für anti-NEFM Antikörper (ABIN94435)

Dráberová, Sulimenko, Kukharskyy, Dráber: "Monoclonal antibody NF-09 specific for neurofilament protein NF-M." in: Folia biologica, Vol. 45, Issue 4, pp. 163-5, (2000) (PubMed).

Details zu NEFM

Target: NEFM (Neurofilament Triplet M Protein (NEFM))

Andere Bezeichnung: Neurofilament M

Hintergrund: Neurofilament medium,Neurofilaments (NFs) are a type of intermediate filament (IF) expressed almost exclusively in neuronal cells, and in those cells most prominently in large axons. NFs in most vertebrates are composed of three different polypeptide chains with different molecular weights –, neurofilament heavy protein (NF-H), medium (NF-M) and light protein (NF-L), which share sequence and structural similarity in a coiled-coil core domain, but differ in the length and sequence of their N-termini and more dramatically of their C-termini which in the case of NF-M and NF-H form the flexible extensions that link NFs to each other and to other elements in the cytoplasm. The protein segment on the C-terminal side of the human NF-H rod is uniquely long (more than 600 amino acids) compared to other IF proteins and is highly charged (> 24 % Glu, > 25 % Lys), rich in proline (> 12 %) and improverished in cysteine, methionine and aromatic amino acids. Its most remarkable feature is a repetitive sequence that covers more than half its lenght and includes the sekvence motif Lys-Ser-Pro (KSP) greater than 40 times. Plasma neurofilament heavy chain level has been proposed as a marker of axonal injury and clinical use of its degeneration and loss has been suggested as a biomarker of several neurodegenerative diseases.,NFM, NEFM, Neurofilament medium chain, NEF3

Gen-ID: 4741

UniProt: P07197

Pathways: Brown Fat Cell Differentiation