Ig Light Chains Antikörper
Kurzübersicht für Ig Light Chains Antikörper (ABIN94401)
Target
Reaktivität
Wirt
Klonalität
Konjugat
Applikation
Klon
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Verwendungszweck
- Anti-Bov Ig Light Chains Purified
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Spezifität
- The antibody IVA285-1 reacts with bovine Ig (light chains). Ig light chains (kappa and lambda types) consist of a variable region and a constant region and can easily be differentiated by the antigenic properties of the constant region.
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Kreuzreaktivität (Details)
- Bovine, Sheep
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Aufreinigung
- Purified by protein-A affinity chromatography.
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Reinheit
- > 95 % (by SDS-PAGE)
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Immunogen
- Fraction of Bovine immunoglobulin.
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Isotyp
- IgG1
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Applikationshinweise
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Western blotting: Recommended dilution: 0,5-1 μg/mL, overnight at 4 °C. Sample preparation: bovine serum, dilution 1:50 in Laemmli reducing buffer, boiled in water bath for 3 min. Reducing conditions, 12 % separating gel. The antibody strongly reacts with bovine IgG light chains, weakly reacts with IgM.
Flow cytometry: Recommended dilution: 1-4 μg/mL -
Beschränkungen
- Nur für Forschungszwecke einsetzbar
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Format
- Liquid
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Konzentration
- 1 mg/mL
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Buffer
- Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
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Konservierungsmittel
- Sodium azide
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Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Handhabung
- Do not freeze.
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Lagerung
- 4 °C
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Informationen zur Lagerung
- Store at 2-8°C. Do not freeze.
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Monoclonal antibody to the light chain of bovine immunoglobulin." in: Hybridoma (2005), Vol. 25, Issue 5, pp. 309-12, (2006) (PubMed).
: "
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Monoclonal antibody to the light chain of bovine immunoglobulin." in: Hybridoma (2005), Vol. 25, Issue 5, pp. 309-12, (2006) (PubMed).
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- Ig Light Chains
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Hintergrund
- Immunoglobulin light chains
Target
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