HLA-E Antikörper

Produktnummer ABIN94356

Zitiert in 6+ Publikationen. Der Maus Monoklonal anti-HLA-E Antikörper (Klon MEM-E-07) (ABIN94356) detektiert spezifisch HLA-E in FACS, IP und IHC (p). Dieser Antikörper reagiert spezifisch mit Proben aus Human.

Kurzübersicht für HLA-E Antikörper (ABIN94356)

Target: HLA-E (HLA Class I Histocompatibility Antigen, alpha Chain E (HLA-E))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser HLA-E Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Klon: MEM-E-07

Produktdetails anti-HLA-E Antikörper

Verwendungszweck: Anti-HLA-E Purified

Kreuzreaktivität (Details): Human

Produktmerkmale: The antibody MEM-E/07 recognizes an extracellular epitope on native surface-expressed HLA-E, but not denaturated heavy chain of HLA-E. HLA-E belongs to the MHC Class I molecules (MHC Class Ib, nonclassical) and it is expressed on many types of the human cells. The published results revealed that the antibody cross-reacts with some classical MHC Class I molecules (MHC Class Ia): HLA-B7 (strongly), HLA-B8 (moderately), HLA-B27, -B44 (weakly).

Aufreinigung: Purified by protein-A affinity chromatography followed by size-exclusion chromatography.

Reinheit: > 95 % (by SDS-PAGE)

Immunogen: Bacterially expressed recombinant HLA-E refolded with beta2-microglobulin and peptide.

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Immunohistochemistry (paraffin sections): Recommended dilution: 10 μg/mL, positive tissue: spleen.
Flow cytometry: Recommended dilution: 1-4 μg/mL

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Do not freeze

Lagerung: 4 °C

Informationen zur Lagerung: Store at 2-8°C. Do not freeze.

Referenzen für anti-HLA-E Antikörper (ABIN94356)

Nachmani, Zimmermann, Oiknine Djian, Weisblum, Livneh, Khanh Le, Galun, Horejsi, Isakov, Shomron, Wolf, Hengel, Mandelboim: "MicroRNA editing facilitates immune elimination of HCMV infected cells." in: PLoS pathogens, Vol. 10, Issue 2, pp. e1003963, (2014) (PubMed).

Allard, Oger, Vignard, Percier, Fregni, Périer, Caignard, Charreau, Bernardeau, Khammari, Dréno, Gervois: "Serum soluble HLA-E in melanoma: a new potential immune-related marker in cancer." in: PLoS ONE, Vol. 6, Issue 6, pp. e21118, (2011) (PubMed).

Lo Monaco, Tremante, Cerboni, Melucci, Sibilio, Zingoni, Nicotra, Natali, Giacomini: "Human leukocyte antigen E contributes to protect tumor cells from lysis by natural killer cells." in: Neoplasia (New York, N.Y.), Vol. 13, Issue 9, pp. 822-30, (2011) (PubMed).

Soni, Karande: "Glycodelin A suppresses the cytolytic activity of CD8+ T lymphocytes." in: Molecular immunology, Vol. 47, Issue 15, pp. 2458-66, (2010) (PubMed).

Das, Long: "Lytic granule polarization, rather than degranulation, is the preferred target of inhibitory receptors in NK cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 185, Issue 8, pp. 4698-704, (2010) (PubMed).

Palmisano, Contardi, Morabito, Gargaglione, Ferrara, Pistillo: "HLA-E surface expression is independent of the availability of HLA class I signal sequence-derived peptides in human tumor cell lines." in: Human immunology, Vol. 66, Issue 1, pp. 1-12, (2004) (PubMed).

Details zu HLA-E

Target: HLA-E (HLA Class I Histocompatibility Antigen, alpha Chain E (HLA-E))

Andere Bezeichnung: HLA-E

Hintergrund: Major histocompatibility complex, class I, E,HLA-E (human leukocyte antigen E) is a non-classical MHC I antigen, which is important for dialogue with NK cells and their regulation through interaction with CD94/NKG2 receptor. Like other MHC I molecules, transmembrane HLA-E molecule (45 kDa) associates with beta2 microglobulin. Unlike HLA-G, expression of HLA-E molecules is not so restricted, but it has been detected at least at mRNA level in virtually all cells and tissues examined. In peripheral blood, HLA-E protein is expressed at least in all mononuclear cells, but in different quantity (B cells and monocytes more than T cells and NK cells).,HLA class I histocompatibility antigen, alpha chain E, MHC class I antigen E

Gen-ID: 3133

UniProt: P13747

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Human Leukocyte Antigen (HLA) in Adaptive Immune Response