Cytokeratin 19 Antikörper

Produktnummer ABIN94290

This anti-Cytokeratin 19 antibody (ABIN94290) is a Mouse Monoclonal antibody detecting Cytokeratin 19 in WB, ELISA, IHC (p), ICC, IP, ICFC. Suitable for Human.

Quick Overview for Cytokeratin 19 Antikörper (ABIN94290)

Target: Cytokeratin 19 (KRT19) (Keratin 19 (KRT19))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Cytokeratin 19 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunoprecipitation (IP), Intracellular Flow Cytometry (ICFC)

Klon: A53-B-A2

Produktdetails anti-Cytokeratin 19 Antikörper

Verwendungszweck: Anti-Cytokeratin 19 Purified

Spezifität: The mouse monoclonal antibody A53-B/A2 recognizes the rod domain of cytokeratin 19 (40 kDa), an intracellular antigen constituting intermmediate cytoskeleton filaments. Cytokeratin 19 is not expressed in hepatocytes, it is often co-expressed with cytokeratin 7.

Kreuzreaktivität (Details): Human

Aufreinigung: Purified by protein-A affinity chromatography.

Reinheit: > 95 % (by SDS-PAGE)

Immunogen: MCF-7 human breast adenocarcinoma cell line

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise: Flow cytometry: Recommended dilution: 1-5 μg/mL. Intracellular staining.
Immunohistochemistry: Recommended dilution: 5-10 μg/mL.
Western blotting: Recommended dilution: 1-2 μg/mL, positive control: MCF-7, BA-17, HT-29 cells.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Do not freeze.

Lagerung: 4 °C

Informationen zur Lagerung: Store at 2-8°C. Do not freeze.

Referenzen für anti-Cytokeratin 19 Antikörper (ABIN94290)

Kasper, Moll, Stosiek, Karsten: "Patterns of cytokeratin and vimentin expression in the human eye." in: Histochemistry, Vol. 89, Issue 4, pp. 369-77, (1988) (PubMed).

Karsten, Papsdorf, Roloff, Stolley, Abel, Walther, Weiss: "Monoclonal anti-cytokeratin antibody from a hybridoma clone generated by electrofusion." in: European journal of cancer & clinical oncology, Vol. 21, Issue 6, pp. 733-40, (1985) (PubMed).

Details zu Cytokeratin 19

Target: Cytokeratin 19 (KRT19) (Keratin 19 (KRT19))

Andere Bezeichnung: Cytokeratin 19

Hintergrund: Keratin 19,Cytokeratins are a subfamily of intermediate filaments and characterized by remarkable biochemical diversity. They are represented in epithelial tissues by at least 20 different polypeptides, molecular weight between 40 kDa and 68 kDa. The individual cytokeratin polypeptides are designated 1 to 20 and divided into the type I (acidic cytokeratins 9-20) and type II (basic to neutral cytokeratins 1-8) families.,K19, CK19, CYK19, KRT19, K1CS

Gen-ID: 3880

UniProt: P08727